| dc.contributor.author |
Zavizion, B |
en |
| dc.contributor.author |
Duffelen, M |
en |
| dc.contributor.author |
Schaeffer, W |
en |
| dc.contributor.author |
Politis, I |
en |
| dc.date.accessioned |
2014-06-06T06:42:56Z |
|
| dc.date.available |
2014-06-06T06:42:56Z |
|
| dc.date.issued |
1995 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1007/BF00986233 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/904 |
|
| dc.subject |
Cell Culture |
en |
| dc.subject |
Cell Line |
en |
| dc.subject |
Epithelial Cell |
en |
| dc.subject |
Mammary Epithelial Cell |
en |
| dc.subject |
Large T Antigen |
en |
| dc.subject |
Stem Cell |
en |
| dc.subject |
Simian Virus |
en |
| dc.title |
Use of microinjection to generate an immortalized bovine mammary cell line with both epithelial and myoepithelial characteristics |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1007/BF00986233 |
en |
| heal.publicationDate |
1995 |
en |
| heal.abstract |
Methods are described for the isolation, purification, and culture of bovine mammary epithelial cells. Individual cells were microinjected with a plasmid bearing the sequence for the simian virus 40 early region mutant tsA58 which encodes the thermolabile large T-antigen. After selection in G418-containing medium, surviving colonies were recovered and recloned. One of the clones, designated (E-TC), has been cultured for |
en |
| heal.journalName |
Journal of Tissue Culture Methods |
en |
| dc.identifier.doi |
10.1007/BF00986233 |
en |