| heal.abstract |
Eleven enzyme activities were used to study the intraspecific diversity and interspecific relatedness of II Pleurotus species: P. abalonus, P. columbinus, P. cornucopiae, P. cystidiosus, P. dryinus, P. eryngii, P. flabellatus, P. ostreatus, P. pulmonarius, P. sajor-caju, and P. sapidus. In addition, the zymograms of their homokaryotic progenies were evaluated to determine the number of genes and alleles encoding them. Among the 18 loci detected, three coded for PEP A, two for G6PD, LAP, MDH, PEP C and PGM, and one for APH, HK, IDH, 6PGD and PHI. Based on electrophoretic patterns, the 77 wild Pleurotus dikaryons were divided into 51 genotypic classes. The higher intraspecific genetic variation was demonstrated by P. cystidiosus and P. eryngii, while P. cornucopiae, P. dryinus and P. sajor-caju appeared to be the least diverse species examined. Furthermore, with the aid of numerical analysis, valid species such as P. cornucopiae, P. dryinus, P. eryngii and P. flabellatus were clearly distinguished. Among the rest of the taxa studied, the derived dendrograms permitted the delimitation between P. abalonus-P. cystidiosus and P. columbinus-P. ostreatus, although the former members of these two pairs were previously considered as varieties. On the other hand P. pulmonarius and P. sajor-caju formed a tight cluster indicative of a certain genetic affinity, which questions their original designation as distinct species. As regards P. sapidus, it presented a slightly higher proximity to the P. pulmonarius-P. sajor-caju complex than to P. ostreatus. Finally, the phylogenetic implications from the findings of this work are discussed. |
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