| dc.contributor.author |
Eiteman, MA |
en |
| dc.date.accessioned |
2014-06-06T06:42:34Z |
|
| dc.date.available |
2014-06-06T06:42:34Z |
|
| dc.date.issued |
1994 |
en |
| dc.identifier.issn |
00219673 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/0021-9673(94)80088-X |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/687 |
|
| dc.subject.other |
1,4 benzenedimethanol |
en |
| dc.subject.other |
1,4 xylylenediamine |
en |
| dc.subject.other |
amine |
en |
| dc.subject.other |
dipeptide |
en |
| dc.subject.other |
macrogol |
en |
| dc.subject.other |
methanol derivative |
en |
| dc.subject.other |
potassium dihydrogen phosphate |
en |
| dc.subject.other |
terephthalic acid |
en |
| dc.subject.other |
unclassified drug |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
mathematical model |
en |
| dc.subject.other |
partition coefficient |
en |
| dc.subject.other |
ph |
en |
| dc.subject.other |
prediction |
en |
| dc.subject.other |
priority journal |
en |
| dc.title |
Predicting partition coefficients of multi-charged solutes in aqueous two-phase systems |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/0021-9673(94)80088-X |
en |
| heal.publicationDate |
1994 |
en |
| heal.abstract |
A mathematical model incorporating the influence of a pH difference between the phases is developed to p}edict the partitioning of charged compounds in aqueous two-phase systems. The partition coefficients of three model compounds, terephthalic acid, benzenedimethanol and xylylenediamine, are examined in poly(ethylene glycol)potassium phosphate aqueous two-phase systems over the pH range 5.5-9.2. The model predicts that in this particular phase system, negatively charged solutes partition higher and positively charged solutes partition lower than an otherwise identical neutral solute. The partition coefficients of the dipeptides tyrosine-tyrosine, glycine-tyrosine and leucine-phenylalanine are also examined in poly(ethylene glycol)-potassium phosphate systems, and their observed behaviour agrees with the model prediction. The addition of an alkali metal halide to a poly(ethylene glycol)-potassium phosphate system was observed to result in a decrease in the pH difference between the phases. As the model predicts for decreasing pH difference between the phases, the observed partition coefficients of negatively charged compounds decrease. The results indicate that charge and hydrophobic effects each play important roles in the partitioning of biological compounds. |
en |
| heal.journalName |
Journal of Chromatography A |
en |
| dc.identifier.issue |
1 |
en |
| dc.identifier.volume |
668 |
en |
| dc.identifier.doi |
10.1016/0021-9673(94)80088-X |
en |
| dc.identifier.spage |
21 |
en |
| dc.identifier.epage |
30 |
en |