| dc.contributor.author |
Tsakalidou, E |
en |
| dc.contributor.author |
Dalezios, I |
en |
| dc.contributor.author |
Georgalaki, M |
en |
| dc.contributor.author |
Kalantzopoulos, G |
en |
| dc.date.accessioned |
2014-06-06T06:42:30Z |
|
| dc.date.available |
2014-06-06T06:42:30Z |
|
| dc.date.issued |
1993 |
en |
| dc.identifier.issn |
00220302 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.3168/jds.S0022-0302(93)77549-9 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/659 |
|
| dc.subject |
Aminopeptidase |
en |
| dc.subject |
Bulgaricus |
en |
| dc.subject |
Lactobacillus delbrueckii ssp |
en |
| dc.subject |
Streptococcus thermophilus |
en |
| dc.subject.other |
Lactobacillus delbrueckii |
en |
| dc.subject.other |
Streptococcus |
en |
| dc.subject.other |
Streptococcus thermophilus |
en |
| dc.title |
A comparative study: Aminopeptidase activities from Lactobaclllus delbrueckii ssp. Bulgaricus and Streptococcus thermophllus |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.3168/jds.S0022-0302(93)77549-9 |
en |
| heal.publicationDate |
1993 |
en |
| heal.abstract |
Aminopeptidases from Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilus were isolated. Enzymes were purified by chromatography on DEAE-cellulose and Sephadex G-150. The enzymes had molecular weights of 98,000 and 89,000 and optimal activity at pH 6.0 and 40°C and pH 6.5 and 35°C, respectively. The L delbrueckii ssp. bulgaricus enzyme had higher activity on L-lysyl-4-nitroanilide than did the 5. thermophilus enzyme. Both enzymes were inactivated by EDTA and 1,10-phenanthroline. Classical sulfhydryl and serine group reagents had little or no inhibitory effect on the enzymes; nevertheless, Cu ++ and Hg ++ resulted in strong inhibition; Ca ++ stimulated the L. delbrueckii ssp. bulgaricus enzyme, and Mg ++ stimulated the S. thermophilus enzyme. |
en |
| heal.journalName |
Journal of Dairy Science |
en |
| dc.identifier.issue |
8 |
en |
| dc.identifier.volume |
76 |
en |
| dc.identifier.doi |
10.3168/jds.S0022-0302(93)77549-9 |
en |
| dc.identifier.spage |
2145 |
en |
| dc.identifier.epage |
2151 |
en |