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Performance of two potential probiotic Lactobacillus strains from the olive microbiota as starters in the fermentation of heat shocked green olives

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dc.contributor.author Argyri, AA en
dc.contributor.author Nisiotou, AA en
dc.contributor.author Mallouchos, A en
dc.contributor.author Panagou, EZ en
dc.contributor.author Tassou, CC en
dc.date.accessioned 2014-06-06T06:53:06Z
dc.date.available 2014-06-06T06:53:06Z
dc.date.issued 2014 en
dc.identifier.issn 01681605 en
dc.identifier.uri http://dx.doi.org/10.1016/j.ijfoodmicro.2013.11.003 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/6369
dc.subject Green olives en
dc.subject L. pentosus en
dc.subject L. plantarum en
dc.subject Olive fermentation en
dc.subject Probiotic bacteria en
dc.subject.other probiotic agent en
dc.subject.other article en
dc.subject.other bacterial strain en
dc.subject.other controlled study en
dc.subject.other fermentation en
dc.subject.other fermentation medium en
dc.subject.other heat shock en
dc.subject.other heat treatment en
dc.subject.other inoculation en
dc.subject.other Lactobacillus pentosus en
dc.subject.other Lactobacillus plantarum en
dc.subject.other microflora en
dc.subject.other mixed cell culture en
dc.subject.other nonhuman en
dc.subject.other olive en
dc.subject.other pH en
dc.subject.other physical chemistry en
dc.subject.other pulsed field gel electrophoresis en
dc.subject.other starter culture en
dc.subject.other strain difference en
dc.subject.other survival rate en
dc.title Performance of two potential probiotic Lactobacillus strains from the olive microbiota as starters in the fermentation of heat shocked green olives en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.ijfoodmicro.2013.11.003 en
heal.publicationDate 2014 en
heal.abstract The performance of two potential probiotic Lactobacillus strains from olive microbiota, namely L. pentosus B281 and L. plantarum B282 was assessed as starter cultures in Spanish-style fermentation of heat shocked green olives cv. Halkidiki. Two different initial salt levels were studied, 10% (w/v) and 8% (w/v) NaCl, and the brines were inoculated with (a) L. pentosus B281, (b) L. plantarum B282, and (c) a mixture of both strains. A spontaneous fermentation was also taken into account as control treatment. Prior to brining, olives were heat shocked at 80. °C for 10. min to reduce the level of the indigenous microbiota on olive drupes and facilitate the dominance of the inoculated cultures. Microbiological, physicochemical and sensory analyses were conducted throughout fermentation. The composition of LAB population and the evolution of added inocula were assessed by Pulsed Field Gel Electrophoresis (PFGE). The final population of LAB was maintained above 6. log cycles in olive flesh. Both L. pentosus B281 and L. plantarum B282 were able to dominate over indigenous LAB, albeit strain B281 exhibited higher recovery percentages (100 or 94.7% for B281 and 58.8% or 55.0% for B282 in 10% or 8% NaCl, respectively). L. pentosus B281 also dominated over L. plantarum B282, when the two strains were co-inoculated in olive fermentations. The sensory assessment showed higher preference for inoculated fermentations of L. pentosus and L. plantarum separately in 8% NaCl, followed by the L. plantarum in 10% NaCl. The present study showed that probiotic strains L. pentosus B281 and L. plantarum B282, may offer a great potential for use as functional starter cultures in olive fermentation and deliver a promising probiotic food to the consumer. © 2013 Elsevier B.V. en
heal.journalName International Journal of Food Microbiology en
dc.identifier.volume 171 en
dc.identifier.doi 10.1016/j.ijfoodmicro.2013.11.003 en
dc.identifier.spage 68 en
dc.identifier.epage 76 en


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