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Gene transcription patterns of pH- and salt-stressed Listeria monocytogenes cells in simulated gastric and pancreatic conditions

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dc.contributor.author Mataragas, M en
dc.contributor.author Greppi, A en
dc.contributor.author Rantsiou, K en
dc.contributor.author Cocolin, L en
dc.date.accessioned 2014-06-06T06:53:03Z
dc.date.available 2014-06-06T06:53:03Z
dc.date.issued 2014 en
dc.identifier.issn 0362028X en
dc.identifier.uri http://dx.doi.org/10.4315/0362-028X.JFP-13-182 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/6334
dc.title Gene transcription patterns of pH- and salt-stressed Listeria monocytogenes cells in simulated gastric and pancreatic conditions en
heal.type journalArticle en
heal.identifier.primary 10.4315/0362-028X.JFP-13-182 en
heal.publicationDate 2014 en
heal.abstract A Listeria monocytogenes subgenomic array, targeting 54 genes involved in the adhesion, adaptation, intracellular life cycle, invasion, and regulation of the infection cycle was used to investigate the gene expression patterns of acid- and salt-stressed Listeria cells after exposure to conditions similar to those in gastric and pancreatic fluids. Three L. monocytogenes strains, one laboratory reference strain (EGDe) and two food isolates (wild strain 12 isolated from milk and wild strain 3 isolated from fermented sausage), were used during the studies. Differences in the expressed genes were observed between the gastric and pancreatic treatments and also between the serotypes. Increased transcripts were observed of the genes belonging to the adaptation and regulation group for serotype 4b (strain 12) and to the invasion and regulation group for serotype 1/2a (strain EGDe). Interestingly, no significantly differentially expressed genes were found for serotype 3c (strain 3) in most cases. The genes related to adaptation (serotype 1/2a) and to intracellular life cycle and invasion (serotype 4b) were down-regulated in order to cope with the hostile environment of the gastric and pancreatic fluids. These findings may provide experimental evidence for the dominance of serotypes 1/2a and 4b in clinical cases of listeriosis and for the sporadic occurrence of serotype 3c. © International Association for Food Protection. en
heal.journalName Journal of Food Protection en
dc.identifier.issue 2 en
dc.identifier.volume 77 en
dc.identifier.doi 10.4315/0362-028X.JFP-13-182 en
dc.identifier.spage 254 en
dc.identifier.epage 261 en


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