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FTIR spectroscopic evaluation of changes in the cellular biochemical composition of the phytopathogenic fungus Alternaria alternata induced by extracts of some Greek medicinal and aromatic plants

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dc.contributor.author Skotti, E en
dc.contributor.author Kountouri, S en
dc.contributor.author Bouchagier, P en
dc.contributor.author Tsitsigiannis, DI en
dc.contributor.author Polissiou, M en
dc.contributor.author Tarantilis, PA en
dc.date.accessioned 2014-06-06T06:53:03Z
dc.date.available 2014-06-06T06:53:03Z
dc.date.issued 2014 en
dc.identifier.issn 13861425 en
dc.identifier.uri http://dx.doi.org/10.1016/j.saa.2014.02.113 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/6333
dc.subject Alternaria alternata en
dc.subject FTIR en
dc.subject Growth enhancement en
dc.subject Medicinal and aromatic plants en
dc.subject.other Amides en
dc.subject.other Aromatic compounds en
dc.subject.other Aromatization en
dc.subject.other Bioactivity en
dc.subject.other Cell membranes en
dc.subject.other Cytology en
dc.subject.other Essential oils en
dc.subject.other Fourier transform infrared spectroscopy en
dc.subject.other Fungi en
dc.subject.other Principal component analysis en
dc.subject.other Proteins en
dc.subject.other Alternaria alternata en
dc.subject.other Aromatic plants en
dc.subject.other Biochemical composition en
dc.subject.other Cellular constituents en
dc.subject.other FTIR en
dc.subject.other Growth enhancement en
dc.subject.other Phytopathogenic fungi en
dc.subject.other Spectroscopic evaluation en
dc.subject.other Plant extracts en
dc.title FTIR spectroscopic evaluation of changes in the cellular biochemical composition of the phytopathogenic fungus Alternaria alternata induced by extracts of some Greek medicinal and aromatic plants en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.saa.2014.02.113 en
heal.publicationDate 2014 en
heal.abstract In this study, the biological activity of aquatic extracts of selected Greek medicinal and aromatic plants to the phytopathogenic fungus Alternaria alternata was investigated. Lamiaceae species (Hyssopus officinalis L., Melissa officinalis L., Origanum dictamnus L., Origanum vulgare L. and Salvia officinalis L.) were found to enhance significantly the mycelium growth whereas Crocus sativus appears to inhibit it slightly. M. officinalis and S. officinalis caused the highest stimulation in mycelium growth (+97%) and conidia production (+65%) respectively. In order to further investigate the bioactivity of plant extracts to A. alternata, we employed Fourier Transform Infrared Spectroscopy (FTIR). Differences of original spectra were assigned mainly to amides of proteins. The second derivative transformation of spectra revealed changes in spectral regions corresponding to absorptions of the major cellular constituents such as cell membrane and proteins. Principal component analysis of the second derivative transformed spectra confirmed that fatty acids of the cell membranes, amides of proteins and polysaccharides of the cell wall had the major contribution to data variation. FTIR band area ratios were found to correlate with fungal mycelium growth. © 2014 Elsevier B.V. All rights reserved. en
heal.publisher Elsevier en
heal.journalName Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy en
dc.identifier.volume 127 en
dc.identifier.doi 10.1016/j.saa.2014.02.113 en
dc.identifier.spage 463 en
dc.identifier.epage 472 en


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