Biodiversity and ITS-RFLP characterisation of Aspergillus section Nigri isolates in grapes from four traditional grape-producing areas in Greece

dc.contributor.advisor	Κιζής, Δημοσθένης	el
dc.contributor.advisor	Kizis, Dimosthenis	en
dc.contributor.author	Natskoulis, Pantelis	en
dc.contributor.author	Nychas, Georgios John E.	en
dc.contributor.author	Panagou, Efstathios Z.	en
dc.contributor.author	Νατσκούλης, Παντελής	el
dc.contributor.author	Νυχάς, Γεώργιος Ιωάννης Ε.	el
dc.contributor.author	Πανάγου, Ευστάθιος Ζ.	el
dc.date.accessioned	2014-06-06T06:52:59Z
dc.date.available	2014-06-06T06:52:59Z
dc.date.issued	2014-04-07	en
dc.identifier.issn	19326203	en
dc.identifier.uri	http://dx.doi.org/10.1371/journal.pone.0093923	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/6287
dc.rights	CC0 1.0 Παγκόσμια	el
dc.rights.uri	http://creativecommons.org/publicdomain/zero/1.0/	en
dc.title	Biodiversity and ITS-RFLP characterisation of Aspergillus section Nigri isolates in grapes from four traditional grape-producing areas in Greece	en
heal.type	journalArticle	en
heal.keyword	Aspergillus	en
heal.keyword	Nigri	en
heal.keyword	Grapes	en
heal.keyword	Greece	en
heal.keyword	Polymerase Chain Reaction	en
heal.keyword	Genetic polymorphism	en
heal.identifier.primary	10.1371/journal.pone.0093923	en
heal.identifier.secondary	e93923	en
heal.recordProvider	Γεωπονικό Πανεπιστήμιο Αθηνών/Επιστήμη Τροφίμων και Διατροφής Ανθρώπου	el
heal.publicationDate	2014-04-07	en
heal.bibliographicCitation	Dimosthenis Kizis. Biodiversity and ITS-RFLP characterisation of Aspergillus section Nigri isolates in grapes from four traditional grape-producing areas in Greece, PLoS One, 9 (4) pp 1-15, PLoS 2014	en
heal.abstract	A study on the occurrence of Aspergillus section Nigri species on grapes from four traditional grape-producing areas in Greece during the 2011/2012 vintage, and their capability to produce OTA was conducted. One hundred and twenty-eight black aspergilli isolates were characterised at the species level initially by the use of morphological criteria in accordance with appropriate keys, followed by molecular characterisation performed with Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) of the 5.8 ribosomal RNA gene Internal Transcribed Spacer region (5.8 rRNA ITS). Restriction enzyme digestion of the ITS amplicons using the HhaI, HinfI and RsaI, endonucleases distinguished eleven different patterns of restriction fragment length polymorphism (RFLP), four for each of the HhaI and Rsa I digests and three for HinfI. From a total number of 128 individual isolates, 124 were classified into four Aspergillus species corresponding to A. carbonarius, A. tubingensis, A. japonicus and A. ibericus, and the remaining 4 were classified as members of the A. niger aggregate. A. carbonarius and A. tubingensis being the main representative species were equally counted, with higher geographical representation of the former in southern and the latter in northern regions, respectively. All isolates were tested for their ochratoxigenic potential by use of High Performance Liquid Chromatography (HPLC) and Enzyme Linked Immuno Sorbent Assay (ELISA), resulting in significant interspecies differences in OTA production.	en
heal.publisher	Public Library of Science (PLoS)	en
heal.journalName	PLoS ONE	en
heal.journalType	Open Access	en
dc.identifier.doi	10.1371/journal.pone.0093923	en