Bacterial reporter strains for d-xylose content analysis in arabinoxylans

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dc.contributor.author Lukasiak, J en
dc.contributor.author Olsen, K en
dc.contributor.author Georgiou, CA en
dc.contributor.author Georgakopoulos, DG en
dc.date.accessioned 2014-06-06T06:52:59Z
dc.date.available 2014-06-06T06:52:59Z
dc.date.issued 2014 en
dc.identifier.issn 14382377 en
dc.identifier.uri http://dx.doi.org/10.1007/s00217-013-2102-y en
dc.subject Arabinoxylan en
dc.subject d-xylose en
dc.subject Fluorescence en
dc.subject Ice nucleation en
dc.subject Microbial biosensor en
dc.subject.other Analytical method en
dc.subject.other Arabinoxylans en
dc.subject.other D-Xylose en
dc.subject.other High-performance anion-exchange chromatography en
dc.subject.other Ice nucleation en
dc.subject.other Length adjustment en
dc.subject.other Microbial biosensor en
dc.subject.other Pseudomonas syringae en
dc.subject.other Chromatography en
dc.subject.other Escherichia coli en
dc.subject.other Fluorescence en
dc.subject.other Genes en
dc.subject.other Nucleation en
dc.subject.other Xylose en
dc.title Bacterial reporter strains for d-xylose content analysis in arabinoxylans en
heal.type journalArticle en
heal.identifier.primary 10.1007/s00217-013-2102-y en
heal.publicationDate 2014 en
heal.abstract A pair of ice nucleation and fluorescence reporter strains induced specifically by d-xylose were developed and optimized, to monitor d-xylose content in hydrolyzed arabinoxylans samples. Reporter strains were developed by fusing the Escherichia coli xylose isomerase promoter P xylA to the promoterless native inaZ gene of Pseudomonas syringae and synthetic gfp gene of Aequorea victoria and transferring final constructs to E. coli DH5α. Because of relatively low initial response toward the target analyte, signal improvement by promoter region length adjustment has been implemented. In both cases, this approach proved to be successful, although the manner in which reporter strains have responded to it was dissimilar. The specificity and quantitative response of obtained reporter strains have been confirmed, and the response ranges for NIxylA100 and gfpxylA300 have been established as 9 × 10-6-9 × 10-1 g l-1 and 9 × 10-3-9 g l-1, respectively. The performance of developed reporter strains has been assessed in comparison with high-performance anion-exchange chromatography and demonstrated 15-18 % difference between data obtained with reporter strains and chromatography analysis, which for microbial sensors is an acceptable dissimilarity. The developed microbial reporter strains present an alternative for other analytical methods used for monosaccharide quantification and enable quantification of d-xylose in arabinoxylans samples. © 2013 Springer-Verlag Berlin Heidelberg. en
heal.journalName European Food Research and Technology en
dc.identifier.issue 2 en
dc.identifier.volume 238 en
dc.identifier.doi 10.1007/s00217-013-2102-y en
dc.identifier.spage 275 en
dc.identifier.epage 283 en

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