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Production of fermentation feedstock from jerusalem artichoke tubers and its potential for polyhydroxybutyrate synthesis

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dc.contributor.author Koutinas, AA en
dc.contributor.author Garcia, IL en
dc.contributor.author Kopsahelis, N en
dc.contributor.author Papanikolaou, S en
dc.contributor.author Webb, C en
dc.contributor.author Villar, MA en
dc.contributor.author Lopez, JA en
dc.date.accessioned 2014-06-06T06:52:46Z
dc.date.available 2014-06-06T06:52:46Z
dc.date.issued 2013 en
dc.identifier.issn 18772641 en
dc.identifier.uri http://dx.doi.org/10.1007/s12649-012-9154-2 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/6163
dc.subject Aspergillus awamori en
dc.subject Bioprocess en
dc.subject Generic fermentation media en
dc.subject Jerusalem artichoke en
dc.subject Polyhydroxybutyrate en
dc.subject Solid state fermentation en
dc.subject.other Aspergillus awamori en
dc.subject.other Bioprocesses en
dc.subject.other Fermentation media en
dc.subject.other Jerusalem artichoke en
dc.subject.other Polyhydroxybutyrate en
dc.subject.other Solid-state fermentation en
dc.subject.other Amino acids en
dc.subject.other Aspergillus en
dc.subject.other Enzymes en
dc.subject.other Feedstocks en
dc.subject.other Fructose en
dc.subject.other Glucose en
dc.subject.other Hydrolysis en
dc.subject.other Macromolecules en
dc.subject.other Polysaccharides en
dc.subject.other Substrates en
dc.subject.other Fermentation en
dc.title Production of fermentation feedstock from jerusalem artichoke tubers and its potential for polyhydroxybutyrate synthesis en
heal.type journalArticle en
heal.identifier.primary 10.1007/s12649-012-9154-2 en
heal.publicationDate 2013 en
heal.abstract A conceptual bioprocessing strategy has been developed for the production of a generic fermentation feedstock from Jerusalem artichoke (JA) tubers. Ground JA tubers were used as substrate in solid state fermentations (SSF) of Aspergillus awamori aiming at the simultaneous production of crude enzymes (e.g. inulinase, invertase, protease) that could be employed in the hydrolysis of JA meal macromolecules. After 48 h SSF, remaining solids that contained the crude enzymes were transferred to an aqueous solution of ground JA tubers for the hydrolysis of major macromolecules (inulin and protein) into assimilable nutrients (e.g. fructose, glucose, amino acids and peptides). Conversions of up to 70 % (w/w) inulin to fructose and glucose, among the highest reported in the literature, were achieved. This bioprocess could be employed for the production of fructose- and glucose-based fermentation media from JA tubers. Crude JA hydrolysates were tested as fermentation media for PHB production in shake flask fermentation resulting in intracellular PHB content up to 51.9 % and PHB concentrations up to 4 g L-1. © 2012 Springer Science+Business Media B.V. en
heal.journalName Waste and Biomass Valorization en
dc.identifier.issue 2 en
dc.identifier.volume 4 en
dc.identifier.doi 10.1007/s12649-012-9154-2 en
dc.identifier.spage 359 en
dc.identifier.epage 370 en


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