| dc.contributor.author |
Malandrakis, AA |
en |
| dc.contributor.author |
Markoglou, AN |
en |
| dc.contributor.author |
Konstantinou, S |
en |
| dc.contributor.author |
Doukas, EG |
en |
| dc.contributor.author |
Kalampokis, JF |
en |
| dc.contributor.author |
Karaoglanidis, GS |
en |
| dc.date.accessioned |
2014-06-06T06:52:42Z |
|
| dc.date.available |
2014-06-06T06:52:42Z |
|
| dc.date.issued |
2013 |
en |
| dc.identifier.issn |
01681605 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.ijfoodmicro.2013.01.014 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/6132 |
|
| dc.subject |
Benzimidazole resistance |
en |
| dc.subject |
Blue mold |
en |
| dc.subject |
Citrinin |
en |
| dc.subject |
Patulin |
en |
| dc.subject |
Penicillium expansum |
en |
| dc.subject.other |
antifungal agent |
en |
| dc.subject.other |
benomyl |
en |
| dc.subject.other |
benzimidazole |
en |
| dc.subject.other |
beta tubulin |
en |
| dc.subject.other |
carbendazim |
en |
| dc.subject.other |
citrinin |
en |
| dc.subject.other |
cyprodinil |
en |
| dc.subject.other |
enilconazole |
en |
| dc.subject.other |
fludioxonil |
en |
| dc.subject.other |
iprodione |
en |
| dc.subject.other |
mycotoxin |
en |
| dc.subject.other |
patulin |
en |
| dc.subject.other |
pyraclostrobin |
en |
| dc.subject.other |
pyrrole derivative |
en |
| dc.subject.other |
tiabendazole |
en |
| dc.subject.other |
tubeconazole |
en |
| dc.subject.other |
unclassified drug |
en |
| dc.subject.other |
amino acid substitution |
en |
| dc.subject.other |
antibiotic resistance |
en |
| dc.subject.other |
antifungal resistance |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
bacterium isolate |
en |
| dc.subject.other |
broth dilution |
en |
| dc.subject.other |
chemosensitivity |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
cross resistance |
en |
| dc.subject.other |
culture medium |
en |
| dc.subject.other |
DNA sequence |
en |
| dc.subject.other |
fungal gene |
en |
| dc.subject.other |
fungus isolation |
en |
| dc.subject.other |
genetic analysis |
en |
| dc.subject.other |
mycelial growth |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
pathogenicity |
en |
| dc.subject.other |
Penicillium expansum |
en |
| dc.subject.other |
phenotype |
en |
| dc.subject.other |
point mutation |
en |
| dc.subject.other |
sequence analysis |
en |
| dc.subject.other |
sporogenesis |
en |
| dc.subject.other |
Aminoimidazole Carboxamide |
en |
| dc.subject.other |
Benzimidazoles |
en |
| dc.subject.other |
Carbamates |
en |
| dc.subject.other |
Dioxoles |
en |
| dc.subject.other |
Drug Resistance, Multiple, Fungal |
en |
| dc.subject.other |
Fruit |
en |
| dc.subject.other |
Fungicides, Industrial |
en |
| dc.subject.other |
Hydantoins |
en |
| dc.subject.other |
Malus |
en |
| dc.subject.other |
Mycotoxins |
en |
| dc.subject.other |
Patulin |
en |
| dc.subject.other |
Penicillium |
en |
| dc.subject.other |
Pyrazoles |
en |
| dc.subject.other |
Pyrimidines |
en |
| dc.subject.other |
Pyrroles |
en |
| dc.subject.other |
Thiabendazole |
en |
| dc.subject.other |
Triazoles |
en |
| dc.subject.other |
Tubulin |
en |
| dc.title |
Molecular characterization, fitness and mycotoxin production of benzimidazole-resistant isolates of Penicillium expansum |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.ijfoodmicro.2013.01.014 |
en |
| heal.publicationDate |
2013 |
en |
| heal.abstract |
Penicillium expansum field-strains resistant to benzimidazole fungicides were isolated in high frequency from decayed apple fruit collected from packinghouses and processing industries located in the region of Imathia, N. Greece. In vitro fungitoxicity tests resulted in the identification of two different resistant phenotypes: highly (BEN-HR) and moderately (BEN-MR) carbendazim-resistant. Thirty seven percent of the isolated P. expansum strains belonged to the BEN-HR phenotype, carried no apparent fitness penalties and exhibited resistance levels higher than 60 based on EC50 values. Cross resistance studies with other benzimidazole fungicides showed that all BEN-HR and BEN-MR isolates were also less sensitive to benomyl and thiabendazole. Fungitoxicity tests on the response of BEN-HR isolates to fungicides belonging to other chemical classes revealed no cross-resistance relationships between benzimidazoles and the phenylpyrrole fludioxonil, the dicarboximide iprodione, the anilinopyrimidine cyprodinil, the QoI pyraclostrobin, the imidazole imazalil and the triazole tebuconazole, indicating that a target-site modification is probably responsible for the BEN-HR phenotype observed. Contrary to the above, some BEN-MR isolates exhibited an increased sensitivity to cyprodinil compared to benzimidazole-sensitive ones. BEN-MR isolates had fitness parameters similar to the benzimidazole-sensitive isolates except for conidia production which appeared significantly decreased. Analysis of mycotoxin production (patulin and citrinin) showed that all benzimidazole-resistant isolates produced mycotoxins at concentrations significantly higher than sensitive isolates both on culture medium and on artificially inoculated apple fruit. Comparison of the β-tubulin gene DNA sequence between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in most but not all HR isolates tested. Molecular analysis of the β-tubulin gene in moderately resistant isolates did not reveal any amino acid substitution. This is the first report on the existence and distribution of highly mycotoxigenic field isolates of P. expansum resistant to the benzimidazoles indicating a high potential risk of increased mycotoxin contamination of pome fruit and by-products. © 2013 Elsevier B.V. |
en |
| heal.journalName |
International Journal of Food Microbiology |
en |
| dc.identifier.issue |
3 |
en |
| dc.identifier.volume |
162 |
en |
| dc.identifier.doi |
10.1016/j.ijfoodmicro.2013.01.014 |
en |
| dc.identifier.spage |
237 |
en |
| dc.identifier.epage |
244 |
en |