| heal.abstract |
The aim of the work was to develop an efficient protocol for micropropagation of the rare and endangered in Greece ×Malosorbus florentina (Zucc.) Browicz. The effect of explant origin (adult and juvenile) and culture medium was investigated at all stages of micropropagation (in vitro culture initiation, proliferation, rooting, ex vitro acclimatization). In vitro cultures initiated at a low percentage (0-33%) from shoot tip explants excised from adult plants on MS medium with 1.0 BA/0.1 IBA (mg/L) due to extended contamination, browning and necrosis of the explants. Multiplication rates of cultures were generally low, except for a culture established in March '04. Shoot tip explants from sprouts of burned plants were also established at low percentages (36% average), but the subcultures showed high multiplication rates. The increase of BA concentration in the medium up to 4.0 mg/L resulted in the production of more shoots of shorter length, while the exclusion of IBA decreased both shoot number and length. Microshoots regenerated from sprouts rooted at a higher percentage and gave more and shorter roots than microshoots from adult plants. Microplants acclimatized ex vitro equally well (60-69%) in media of peat-perlite 1:1 or 1:2 (v/v) or plain perlite, but plantlets grew taller in peat-perlite 1:1 compared to plain perlite. The adult or juvenile origin of the microplants did not affect the acclimatization percentage and plantlet height, but plantlets from juvenile origin formed more nodes. © ISHS 2013. |
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