| dc.contributor.author |
Amarowicz, R |
en |
| dc.contributor.author |
Pegg, RB |
en |
| dc.date.accessioned |
2014-06-06T06:52:38Z |
|
| dc.date.available |
2014-06-06T06:52:38Z |
|
| dc.date.issued |
2013 |
en |
| dc.identifier.issn |
17564646 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.jff.2013.01.009 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/6098 |
|
| dc.subject |
Anticancer activity |
en |
| dc.subject |
Arctostaphylos uva-ursi |
en |
| dc.subject |
Bearberry leaves |
en |
| dc.subject |
Carcinoma cells |
en |
| dc.subject |
Phenolics |
en |
| dc.subject |
Proliferation |
en |
| dc.subject.other |
Arctostaphylos uva-ursi |
en |
| dc.title |
Inhibition of proliferation of human carcinoma cell lines by phenolic compounds from a bearberry-leaf crude extract and its fractions |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.jff.2013.01.009 |
en |
| heal.publicationDate |
2013 |
en |
| heal.abstract |
Phenolic compounds were extracted from the leaves of bearberry, a potential functional food ingredient, using 80% (v/v) aqueous ethanol after which the resultant crude extract was applied to a Sephadex LH-20 column. A fraction comprising low-molecular-weight phenolics (LMW fraction) and sugars was eluted from the column with 95% (v/v) ethanol. A tannin fraction was then obtained after switching the mobile phase to acetone/water (1:1, v/v). Phenolic compounds present in the crude extract and its two fractions showed antiproliferative activities in a concentration-dependent manner against five carcinoma cell lines, namely MCF-7 (estrogen receptor-positive breast carcinoma), DU-145 (androgen receptor-negative prostate carcinoma), HT-29 (colon carcinoma), SK-MEL-5 and MDA-MB-435 (melanoma; skin carcinoma). IC50 data revealed that the tannin fraction was best at retarding cell proliferation in the tested cancer cell lines. The greatest inhibition at 1.5μg fraction/mL assay was observed for the HT-29 colon carcinoma cell line. The proliferation of SK-MEL-5 skin carcinoma cells was also strongly inhibited by both the crude extract and LMW fraction. MDA-MB-435 cells were found to be the least sensitive for the materials tested, particularly for the LMW fraction. © 2013 Elsevier Ltd. |
en |
| heal.journalName |
Journal of Functional Foods |
en |
| dc.identifier.issue |
2 |
en |
| dc.identifier.volume |
5 |
en |
| dc.identifier.doi |
10.1016/j.jff.2013.01.009 |
en |
| dc.identifier.spage |
660 |
en |
| dc.identifier.epage |
667 |
en |