| dc.contributor.author |
Taka, S |
en |
| dc.contributor.author |
Liandris, E |
en |
| dc.contributor.author |
Gazouli, M |
en |
| dc.contributor.author |
Sotirakoglou, K |
en |
| dc.contributor.author |
Theodoropoulos, G |
en |
| dc.contributor.author |
Bountouri, M |
en |
| dc.contributor.author |
Andreadou, M |
en |
| dc.contributor.author |
Ikonomopoulos, J |
en |
| dc.date.accessioned |
2014-06-06T06:52:38Z |
|
| dc.date.available |
2014-06-06T06:52:38Z |
|
| dc.date.issued |
2013 |
en |
| dc.identifier.issn |
15671348 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.meegid.2013.03.033 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/6091 |
|
| dc.subject |
SLC11A1 MAP goat |
en |
| dc.subject.other |
interleukin 10 |
en |
| dc.subject.other |
interleukin 1alpha |
en |
| dc.subject.other |
messenger RNA |
en |
| dc.subject.other |
natural resistance associated macrophage protein 1 |
en |
| dc.subject.other |
3' untranslated region |
en |
| dc.subject.other |
animal cell |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
breeding line |
en |
| dc.subject.other |
gene expression |
en |
| dc.subject.other |
gene function |
en |
| dc.subject.other |
gene sequence |
en |
| dc.subject.other |
genetic polymorphism |
en |
| dc.subject.other |
genotype |
en |
| dc.subject.other |
goat |
en |
| dc.subject.other |
homozygote |
en |
| dc.subject.other |
immune response gene |
en |
| dc.subject.other |
in vitro study |
en |
| dc.subject.other |
interleukin 10 gene |
en |
| dc.subject.other |
interleukin 1alpha gene |
en |
| dc.subject.other |
macrophage |
en |
| dc.subject.other |
microsatellite marker |
en |
| dc.subject.other |
monocyte |
en |
| dc.subject.other |
Mycobacterium paratuberculosis |
en |
| dc.subject.other |
natural resistance associated macrophage protein 1 gene |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
nucleotide sequence |
en |
| dc.subject.other |
peripheral blood mononuclear cell |
en |
| dc.subject.other |
primary cell culture |
en |
| dc.subject.other |
priority journal |
en |
| dc.subject.other |
real time polymerase chain reaction |
en |
| dc.subject.other |
regulator gene |
en |
| dc.subject.other |
sequence analysis |
en |
| dc.subject.other |
upregulation |
en |
| dc.subject.other |
3' Untranslated Regions |
en |
| dc.subject.other |
Alleles |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
Base Sequence |
en |
| dc.subject.other |
Cation Transport Proteins |
en |
| dc.subject.other |
Cells, Cultured |
en |
| dc.subject.other |
Gene Expression |
en |
| dc.subject.other |
Genotype |
en |
| dc.subject.other |
Goats |
en |
| dc.subject.other |
Interleukin-10 |
en |
| dc.subject.other |
Interleukin-1alpha |
en |
| dc.subject.other |
Macrophages |
en |
| dc.subject.other |
Molecular Sequence Data |
en |
| dc.subject.other |
Mycobacterium avium subsp. paratuberculosis |
en |
| dc.subject.other |
Polymorphism, Genetic |
en |
| dc.subject.other |
Capra |
en |
| dc.subject.other |
Capra hircus |
en |
| dc.subject.other |
Mycobacterium avium |
en |
| dc.subject.other |
Mycobacterium avium subsp. paratuberculosis |
en |
| dc.title |
In vitro expression of the SLC11A1 gene in goat monocyte-derived macrophages challenged with Mycobacterium avium subsp paratuberculosis |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.meegid.2013.03.033 |
en |
| heal.publicationDate |
2013 |
en |
| heal.abstract |
Johne's disease or paratuberculosis is a chronic, progressive intestinal disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). One of the genes that have been targeted with regard to resistance or sensitivity to paratuberculosis is the SLC11A1 (solute carrier family 11 member A1). Here we extend our previous work to the sequence and structure analysis of the caprine SLC11A1 gene and we assess the functional impact of the most frequent polymorphisms of the 3' UTR region of the SLC11A1 gene to its expression in goat macrophages exposed in vitro to MAP. The role of these polymorphisms in primary immune response is also investigated with connection to gene expression of two interleukins (IL), one of which pro (IL-1a), and the other anti-inflammatory (IL-10). In order to assess gene response, quantitative detection of the SLC11A1, IL-10 and IL1a mRNA was performed by real time PCR before, and at 1, 3 and 24. h after exposure of primary cultures of peripheral blood monocyte-derived macrophages to MAP, collected from 54 goats of the Greek native goat breed. Sequence analysis of the 3' UTR end of the caprine SLC11A1 gene determined its full length to be 522 bases. Structure analysis confirmed the presence of two microsatellites consisted of a variable number of guanine-thymine repeats (regions A and B). The homozygous B7 genotype [B(GTn)7/7] was associated at a statistically significant level with increased expression of the SLC11A1 and IL-1α genes indicating increased in vitro responsiveness and therefore resistance of mononuclear derived macrophages to MAP infection. © 2013 Elsevier B.V. |
en |
| heal.journalName |
Infection, Genetics and Evolution |
en |
| dc.identifier.volume |
17 |
en |
| dc.identifier.doi |
10.1016/j.meegid.2013.03.033 |
en |
| dc.identifier.spage |
8 |
en |
| dc.identifier.epage |
15 |
en |