| dc.contributor.author |
Papanikolaou, S |
en |
| dc.contributor.author |
Beopoulos, A |
en |
| dc.contributor.author |
Koletti, A |
en |
| dc.contributor.author |
Thevenieau, F |
en |
| dc.contributor.author |
Koutinas, AA |
en |
| dc.contributor.author |
Nicaud, J-M |
en |
| dc.contributor.author |
Aggelis, G |
en |
| dc.date.accessioned |
2014-06-06T06:52:38Z |
|
| dc.date.available |
2014-06-06T06:52:38Z |
|
| dc.date.issued |
2013 |
en |
| dc.identifier.issn |
01681656 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.jbiotec.2013.10.025 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/6090 |
|
| dc.subject |
Citric acid |
en |
| dc.subject |
Crude glycerol |
en |
| dc.subject |
Methylcitrate cycle |
en |
| dc.subject |
Oleaginous yeast |
en |
| dc.subject |
Yarrowia lipolytica |
en |
| dc.subject.other |
Biodiesel-derived glycerols |
en |
| dc.subject.other |
Crude glycerol |
en |
| dc.subject.other |
Lipid accumulations |
en |
| dc.subject.other |
Methylcitrate cycle |
en |
| dc.subject.other |
Nitrogen limitation |
en |
| dc.subject.other |
Oleaginous yeasts |
en |
| dc.subject.other |
Wild-type strain |
en |
| dc.subject.other |
Yarrowia lipolytica |
en |
| dc.subject.other |
Citric acid |
en |
| dc.subject.other |
Experiments |
en |
| dc.subject.other |
Glycerol |
en |
| dc.subject.other |
Yeast |
en |
| dc.subject.other |
Nitrogen |
en |
| dc.subject.other |
2 methyl citrate dehydratase |
en |
| dc.subject.other |
biodiesel |
en |
| dc.subject.other |
citric acid |
en |
| dc.subject.other |
fungal enzyme |
en |
| dc.subject.other |
glycerol |
en |
| dc.subject.other |
hydrolyase |
en |
| dc.subject.other |
lipid |
en |
| dc.subject.other |
nitrogen |
en |
| dc.subject.other |
unclassified drug |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
citric acid cycle |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
culture medium |
en |
| dc.subject.other |
dry weight |
en |
| dc.subject.other |
enzyme inactivation |
en |
| dc.subject.other |
enzyme substrate |
en |
| dc.subject.other |
fungal cell |
en |
| dc.subject.other |
fungal metabolism |
en |
| dc.subject.other |
fungal strain |
en |
| dc.subject.other |
fungus culture |
en |
| dc.subject.other |
fungus growth |
en |
| dc.subject.other |
fungus mutant |
en |
| dc.subject.other |
in vivo study |
en |
| dc.subject.other |
lipid storage |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
priority journal |
en |
| dc.subject.other |
wild type |
en |
| dc.subject.other |
Yarrowia lipolytica |
en |
| dc.subject.other |
Yarrowia lipolytica |
en |
| dc.title |
Importance of the methyl-citrate cycle on glycerol metabolism in the yeast Yarrowia lipolytica |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.jbiotec.2013.10.025 |
en |
| heal.publicationDate |
2013 |
en |
| heal.abstract |
A novel approach to trigger lipid accumulation and/or citrate production in vivo through the inactivation of the 2-methyl-citrate dehydratase in Yarrowia lipolytica was developed. In nitrogen-limited cultures with biodiesel-derived glycerol utilized as substrate, the δ. phd1 mutant (JMY1203) produced 57.7. g/L of total citrate, 1.6-fold more than the wild-type strain, with a concomitant glycerol to citrate yield of 0.91. g/g. Storage lipid in cells increased at the early growth stages, suggesting that inactivation of the 2-methyl-citrate dehydratase would mimic nitrogen limitation. Thus, a trial of JMY1203 strain was performed with glycerol under nitrogen-excess conditions. Compared with the equivalent nitrogen-limited culture, significant quantities of lipid (up to ~31% w/w in dry weight, 1.6-fold higher than the nitrogen-limited experiment) were produced. Also, non-negligible quantities of citric acid (up to ~26. g/L, though 0.57-fold lower than the nitrogen-limited experiment) were produced, despite remarkable nitrogen presence into the medium, indicating the construction of phenotype that constitutively accumulated lipid and secreted citrate in Y. lipolytica during growth on waste glycerol utilized as substrate. © 2013 Elsevier B.V. |
en |
| heal.journalName |
Journal of Biotechnology |
en |
| dc.identifier.issue |
4 |
en |
| dc.identifier.volume |
168 |
en |
| dc.identifier.doi |
10.1016/j.jbiotec.2013.10.025 |
en |
| dc.identifier.spage |
303 |
en |
| dc.identifier.epage |
314 |
en |