| dc.contributor.author |
Garcia, IL |
en |
| dc.contributor.author |
Lopez, JA |
en |
| dc.contributor.author |
Dorado, MP |
en |
| dc.contributor.author |
Kopsahelis, N |
en |
| dc.contributor.author |
Alexandri, M |
en |
| dc.contributor.author |
Papanikolaou, S |
en |
| dc.contributor.author |
Villar, MA |
en |
| dc.contributor.author |
Koutinas, AA |
en |
| dc.date.accessioned |
2014-06-06T06:52:31Z |
|
| dc.date.available |
2014-06-06T06:52:31Z |
|
| dc.date.issued |
2013 |
en |
| dc.identifier.issn |
09608524 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.biortech.2012.11.088 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/6053 |
|
| dc.subject |
Crude glycerol |
en |
| dc.subject |
Cupriavidus necator |
en |
| dc.subject |
Microbial bioconversion |
en |
| dc.subject |
Polyhydroxyalkanoates (PHA) |
en |
| dc.subject |
Rapeseed meal hydrolysate |
en |
| dc.subject.other |
Amino nitrogen |
en |
| dc.subject.other |
Bacterial growth |
en |
| dc.subject.other |
Batch fermentation |
en |
| dc.subject.other |
Biodiesel industry |
en |
| dc.subject.other |
Biodiesel plants |
en |
| dc.subject.other |
Biodiesel production |
en |
| dc.subject.other |
Biorefineries |
en |
| dc.subject.other |
Carbon source |
en |
| dc.subject.other |
Crude glycerol |
en |
| dc.subject.other |
Crystallinities |
en |
| dc.subject.other |
Cupriavidus necator |
en |
| dc.subject.other |
Fed-batch fermentation |
en |
| dc.subject.other |
Inhibitory effect |
en |
| dc.subject.other |
NaCl concentration |
en |
| dc.subject.other |
Nutrient supplements |
en |
| dc.subject.other |
PHA production |
en |
| dc.subject.other |
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) |
en |
| dc.subject.other |
Polyhydroxyalkanoates |
en |
| dc.subject.other |
Polyhydroxybutyrate |
en |
| dc.subject.other |
Rapeseed meals |
en |
| dc.subject.other |
Shake flasks |
en |
| dc.subject.other |
Biodiesel |
en |
| dc.subject.other |
Bottles |
en |
| dc.subject.other |
Differential scanning calorimetry |
en |
| dc.subject.other |
Nuclear magnetic resonance spectroscopy |
en |
| dc.subject.other |
Oilseeds |
en |
| dc.subject.other |
Sodium chloride |
en |
| dc.subject.other |
Thermogravimetric analysis |
en |
| dc.subject.other |
Fermentation |
en |
| dc.subject.other |
biodiesel |
en |
| dc.subject.other |
glycerol |
en |
| dc.subject.other |
poly(3 hydroxybutyrate co 3 hydroxyvalerate) |
en |
| dc.subject.other |
polyhydroxyalkanoic acid |
en |
| dc.subject.other |
sodium chloride |
en |
| dc.subject.other |
unclassified drug |
en |
| dc.subject.other |
bacterium |
en |
| dc.subject.other |
biofuel |
en |
| dc.subject.other |
fermentation |
en |
| dc.subject.other |
inhibitor |
en |
| dc.subject.other |
nuclear magnetic resonance |
en |
| dc.subject.other |
polymer |
en |
| dc.subject.other |
sustainability |
en |
| dc.subject.other |
thermogravimetry |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
bacterial growth |
en |
| dc.subject.other |
carbon nuclear magnetic resonance |
en |
| dc.subject.other |
carbon source |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
crystallization |
en |
| dc.subject.other |
Cupriavidus necator |
en |
| dc.subject.other |
differential scanning calorimetry |
en |
| dc.subject.other |
fermentation |
en |
| dc.subject.other |
glass transition temperature |
en |
| dc.subject.other |
melting point |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
oil industry |
en |
| dc.subject.other |
priority journal |
en |
| dc.subject.other |
rapeseed |
en |
| dc.subject.other |
thermogravimetry |
en |
| dc.subject.other |
Biofuels |
en |
| dc.subject.other |
Brassica rapa |
en |
| dc.subject.other |
Cupriavidus necator |
en |
| dc.subject.other |
Fermentation |
en |
| dc.subject.other |
Glycerol |
en |
| dc.subject.other |
Industrial Waste |
en |
| dc.subject.other |
Polyesters |
en |
| dc.subject.other |
Polyhydroxyalkanoates |
en |
| dc.subject.other |
Bacteria (microorganisms) |
en |
| dc.subject.other |
Brassica napus |
en |
| dc.subject.other |
Cupriavidus necator |
en |
| dc.title |
Evaluation of by-products from the biodiesel industry as fermentation feedstock for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production by Cupriavidus necator |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.biortech.2012.11.088 |
en |
| heal.publicationDate |
2013 |
en |
| heal.abstract |
Utilization of by-products from oilseed-based biodiesel production (crude glycerol, rapeseed meal hydrolysates) for microbial polyhydroxyalkanoate (PHA) production could lead to the replacement of expensive carbon sources, nutrient supplements and precursors for co-polymer production. Batch fermentations in shake flasks with varying amounts of free amino nitrogen led to the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV)) with a 2.8-8% 3HV content. Fed-batch fermentations in shake flasks led to the production of 10.9g/L P(3HB-co-3HV) and a 55.6% P(3HB-co-3HV) content. NaCl concentrations between 2 and 6g/L gradually became inhibitory to bacterial growth and PHA formation, whereas in the case of K2SO4, the inhibitory effect was observed only at concentrations higher than 20g/L. Differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and nuclear magnetic resonance (13C NMR) demonstrated that the incorporation of 3HV into the obtained P(3HB-co-3HV) lowered glass transition temperature, crystallinity and melting point as compared to polyhydroxybutyrate. Integrating PHA production in existing oilseed-based biodiesel plants could enhance the viability and sustainability of this first generation biorefinery. © 2012 Elsevier Ltd. |
en |
| heal.journalName |
Bioresource Technology |
en |
| dc.identifier.volume |
130 |
en |
| dc.identifier.doi |
10.1016/j.biortech.2012.11.088 |
en |
| dc.identifier.spage |
16 |
en |
| dc.identifier.epage |
22 |
en |