Evaluating glucose and xylose as cosubstrates for lipid accumulation and γ-linolenic acid biosynthesis of Thamnidium elegans

dc.contributor.author	Zikou, E	en
dc.contributor.author	Chatzifragkou, A	en
dc.contributor.author	Koutinas, AA	en
dc.contributor.author	Papanikolaou, S	en
dc.date.accessioned	2014-06-06T06:52:31Z
dc.date.available	2014-06-06T06:52:31Z
dc.date.issued	2013	en
dc.identifier.issn	13645072	en
dc.identifier.uri	http://dx.doi.org/10.1111/jam.12116	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/6049
dc.subject	γ-linolenic acid	en
dc.subject	Lignocellulosic sugars	en
dc.subject	Lipids	en
dc.subject	Thamnidium elegans	en
dc.subject	Xylitol	en
dc.subject	Xylose	en
dc.subject.other	gamma linolenic acid	en
dc.subject.other	glucose	en
dc.subject.other	lignocellulose	en
dc.subject.other	phospholipid	en
dc.subject.other	sugar	en
dc.subject.other	xylitol	en
dc.subject.other	xylose	en
dc.subject.other	accumulation	en
dc.subject.other	bioreactor	en
dc.subject.other	biotechnology	en
dc.subject.other	cellulose	en
dc.subject.other	cultivation	en
dc.subject.other	fungus	en
dc.subject.other	glucose	en
dc.subject.other	lignin	en
dc.subject.other	lipid	en
dc.subject.other	substrate	en
dc.subject.other	article	en
dc.subject.other	batch reactor	en
dc.subject.other	biomass	en
dc.subject.other	bioreactor	en
dc.subject.other	biosynthesis	en
dc.subject.other	carbon source	en
dc.subject.other	fungus growth	en
dc.subject.other	lipid storage	en
dc.subject.other	lipogenesis	en
dc.subject.other	nonhuman	en
dc.subject.other	Thamnidium	en
dc.subject.other	Thamnidium elegans	en
dc.subject.other	Zygomycetes	en
dc.subject.other	Biomass	en
dc.subject.other	Bioreactors	en
dc.subject.other	Biotechnology	en
dc.subject.other	Carbon	en
dc.subject.other	Fermentation	en
dc.subject.other	gamma-Linolenic Acid	en
dc.subject.other	Glucose	en
dc.subject.other	Industrial Microbiology	en
dc.subject.other	Mucorales	en
dc.subject.other	Nitrogen	en
dc.subject.other	Xylitol	en
dc.subject.other	Xylose	en
dc.subject.other	Fungi	en
dc.subject.other	Thamnidium elegans	en
dc.subject.other	Zygomycetes	en
dc.title	Evaluating glucose and xylose as cosubstrates for lipid accumulation and γ-linolenic acid biosynthesis of Thamnidium elegans	en
heal.type	journalArticle	en
heal.identifier.primary	10.1111/jam.12116	en
heal.publicationDate	2013	en
heal.abstract	Aims: To study the biotechnological production of lipids containing rich amounts of the medically and nutritionally important γ-linolenic acid (GLA), during cultivation of the Zygomycetes Thamnidium elegans, on mixtures of glucose and xylose, abundant sugars of lignocellulosic biomass. Methods and Results: Glucose and xylose were utilized as carbon sources, solely or in mixtures, under nitrogen-limited conditions, in batch-flask or bioreactor cultures. On glucose, T. elegans produced 31·9 g l-1 of biomass containing 15·0 g l-1 lipid with significantly high GLA content (1014 mg l-1). Xylose was proved to be an adequate substrate for growth and lipid production. Additionally, xylitol secretion occurred when xylose was utilized as carbon source, solely or in mixtures with glucose. Batch-bioreactor trials on glucose yielded satisfactory lipid production, with rapid substrate consumption rates. Analysis of intracellular lipids showed that the highest GLA content was observed in early stationary growth phase, while the phospholipid fraction was the most unsaturated fraction of T. elegans. Conclusions: Thamnidium elegans represents a promising fungus for the successful valorization of sugar-based lignocellulosic residues into microbial lipids of high nutritional and pharmaceutical interest. Significance and Impact of the Study: Xylitol production and cultivation in bioreactor trials is reported for the first time for T. elegans, while cultivation on xylose-based media resulted in high GLA production by this fungus. © 2012 The Society for Applied Microbiology.	en
heal.journalName	Journal of Applied Microbiology	en
dc.identifier.issue	4	en
dc.identifier.volume	114	en
dc.identifier.doi	10.1111/jam.12116	en
dc.identifier.spage	1020	en
dc.identifier.epage	1032	en