| dc.contributor.author |
Metsoviti, M |
en |
| dc.contributor.author |
Zeng, A-P |
en |
| dc.contributor.author |
Koutinas, AA |
en |
| dc.contributor.author |
Papanikolaou, S |
en |
| dc.date.accessioned |
2014-06-06T06:52:30Z |
|
| dc.date.available |
2014-06-06T06:52:30Z |
|
| dc.date.issued |
2013 |
en |
| dc.identifier.issn |
01681656 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.jbiotec.2012.11.018 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/6046 |
|
| dc.subject |
1,3-Propanediol |
en |
| dc.subject |
Biodiesel |
en |
| dc.subject |
Citrobacter freundii |
en |
| dc.subject |
Crude glycerol |
en |
| dc.subject |
Non-sterile bioprocess |
en |
| dc.subject.other |
1 ,3 propanediol |
en |
| dc.subject.other |
Batch bioreactors |
en |
| dc.subject.other |
Batch experiments |
en |
| dc.subject.other |
Biodiesel-derived glycerols |
en |
| dc.subject.other |
Bioprocesses |
en |
| dc.subject.other |
Citrobacter freundii |
en |
| dc.subject.other |
Crude glycerol |
en |
| dc.subject.other |
Fed-batch fermentation |
en |
| dc.subject.other |
Fed-batch process |
en |
| dc.subject.other |
Glycerol concentration |
en |
| dc.subject.other |
High substrate concentrations |
en |
| dc.subject.other |
Non-sterile condition |
en |
| dc.subject.other |
Production of |
en |
| dc.subject.other |
Raw glycerol |
en |
| dc.subject.other |
Substrate concentrations |
en |
| dc.subject.other |
Volumetric productivity |
en |
| dc.subject.other |
Waste glycerols |
en |
| dc.subject.other |
Batch data processing |
en |
| dc.subject.other |
Biochemistry |
en |
| dc.subject.other |
Biodiesel |
en |
| dc.subject.other |
Fermentation |
en |
| dc.subject.other |
Oils and fats |
en |
| dc.subject.other |
Substrates |
en |
| dc.subject.other |
Glycerol |
en |
| dc.subject.other |
1,3 propanediol |
en |
| dc.subject.other |
biodiesel |
en |
| dc.subject.other |
glycerol |
en |
| dc.subject.other |
alcohol production |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
bacterial strain |
en |
| dc.subject.other |
bacterium isolation |
en |
| dc.subject.other |
batch reactor |
en |
| dc.subject.other |
biomass |
en |
| dc.subject.other |
bioprocess |
en |
| dc.subject.other |
Citrobacter freundii |
en |
| dc.subject.other |
cooking |
en |
| dc.subject.other |
fed batch culture |
en |
| dc.subject.other |
fed batch fermentation |
en |
| dc.subject.other |
green chemistry |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
priority journal |
en |
| dc.subject.other |
transesterification |
en |
| dc.subject.other |
Batch Cell Culture Techniques |
en |
| dc.subject.other |
Biofuels |
en |
| dc.subject.other |
Bioreactors |
en |
| dc.subject.other |
Biotechnology |
en |
| dc.subject.other |
Citrobacter freundii |
en |
| dc.subject.other |
Culture Media |
en |
| dc.subject.other |
Fermentation |
en |
| dc.subject.other |
Glycerol |
en |
| dc.subject.other |
Propylene Glycols |
en |
| dc.subject.other |
Sterilization |
en |
| dc.subject.other |
Citrobacter freundii |
en |
| dc.title |
Enhanced 1,3-propanediol production by a newly isolated Citrobacter freundii strain cultivated on biodiesel-derived waste glycerol through sterile and non-sterile bioprocesses |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.jbiotec.2012.11.018 |
en |
| heal.publicationDate |
2013 |
en |
| heal.abstract |
The production of 1,3-propanediol (PD) by a newly isolated Citrobacter freundii strain [FMCC-B 294 (VK-19)] was investigated. Different grades of biodiesel-derived glycerol were employed. Slightly lower PD biosynthesis was observed in batch experiments only when crude glycerol from waste-cooking oil trans-esterification was utilized and only at elevated initial substrate concentrations employed. Batch bioreactor cultures revealed the capability of the strain to tolerate elevated amounts of substrate (glycerol up to 170. g/L) and produce quantities of PD in such high substrate concentrations. Nevertheless, maximum PD quantities (45.9. g/L) were achieved at lower initial glycerol concentrations (∼100. g/L) employed, suggesting some inhibition exerted due to the increased initial substrate concentrations. In order to improve PD production, a fed-batch fermentation was carried out and 68.1. g/L of PD were produced (the highest PD quantity achieved by C. freundii strains so far) with yield per glycerol consumed ∼0.40. g/g and volumetric productivity 0.79. g/L/h. Aiming to perform a more economical and eco-friendlier procedure, batch and fed-batch fermentations under completely non-sterile conditions were carried out. During non-sterilized fed-batch process, 176. g/L of raw glycerol were converted to 66.3. g/L of PD, suggesting the potentiality of the non-sterile fermentation by C. freundii FMCC-B 294. © 2012 Elsevier B.V. |
en |
| heal.journalName |
Journal of Biotechnology |
en |
| dc.identifier.issue |
4 |
en |
| dc.identifier.volume |
163 |
en |
| dc.identifier.doi |
10.1016/j.jbiotec.2012.11.018 |
en |
| dc.identifier.spage |
408 |
en |
| dc.identifier.epage |
418 |
en |