Endocrinological profile and follicular development in cyclic ewes subjected to repeated ovum pick-up

dc.contributor.author	Valasi, I	en
dc.contributor.author	Theodosiadou, E	en
dc.contributor.author	Fthenakis, GC	en
dc.contributor.author	Papanikolaou, T	en
dc.contributor.author	Deligiannis, C	en
dc.contributor.author	Kalogiannis, D	en
dc.contributor.author	Chadio, S	en
dc.contributor.author	Amiridis, GS	en
dc.date.accessioned	2014-06-06T06:52:30Z
dc.date.available	2014-06-06T06:52:30Z
dc.date.issued	2013	en
dc.identifier.issn	03784320	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.anireprosci.2013.02.026	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/6044
dc.subject	Ewes	en
dc.subject	Follicular aspiration	en
dc.subject	FSH	en
dc.subject	Oestradiol	en
dc.subject	Oestrous cycle	en
dc.subject	Progesterone	en
dc.subject.other	estradiol	en
dc.subject.other	follitropin	en
dc.subject.other	hormone	en
dc.subject.other	progesterone	en
dc.subject.other	animal	en
dc.subject.other	animal disease	en
dc.subject.other	article	en
dc.subject.other	blood	en
dc.subject.other	cell count	en
dc.subject.other	cell size	en
dc.subject.other	controlled clinical trial	en
dc.subject.other	controlled study	en
dc.subject.other	cytology	en
dc.subject.other	domestic sheep	en
dc.subject.other	estrus cycle	en
dc.subject.other	female	en
dc.subject.other	metabolism	en
dc.subject.other	methodology	en
dc.subject.other	oocyte	en
dc.subject.other	oocyte retrieval	en
dc.subject.other	ovary follicle	en
dc.subject.other	physiology	en
dc.subject.other	randomized controlled trial	en
dc.subject.other	statistics	en
dc.subject.other	Animals	en
dc.subject.other	Cell Count	en
dc.subject.other	Cell Size	en
dc.subject.other	Estradiol	en
dc.subject.other	Estrous Cycle	en
dc.subject.other	Female	en
dc.subject.other	Follicle Stimulating Hormone	en
dc.subject.other	Hormones	en
dc.subject.other	Oocyte Retrieval	en
dc.subject.other	Oocytes	en
dc.subject.other	Ovarian Follicle	en
dc.subject.other	Ovum	en
dc.subject.other	Progesterone	en
dc.subject.other	Sheep, Domestic	en
dc.title	Endocrinological profile and follicular development in cyclic ewes subjected to repeated ovum pick-up	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.anireprosci.2013.02.026	en
heal.publicationDate	2013	en
heal.abstract	Blood concentrations of progesterone, FSH and oestradiol in Karagouniko ewes subjected to laparoscopic ovum pick-up (OPU) at specific stages of induced oestrous cycle, were measured. Twenty-four cyclic ewes were randomly allocated into four equal groups (A, B, C and D). Oestrus was synchronized with progestagen intravaginal sponges and detected by teaser rams (oestrus: day 0). In group A, during the induced oestrous cycle, OPU was performed on days 4, 9 and 14 (sessions A1, A2 and A3, respectively). In group B and group D, OPU was performed once, on day 9 and 14, respectively. In group C (controls), endoscopic observation of follicular population was performed three times, as in group A. Starting at sponge removal, progesterone was measured in blood samples collected on 22 daily occasions and oestradiol in samples collected on 27 occasions collected at various time-points starting 2. h before to 24. h after OPU. Follicular populations did not differ among A1, A2, A3 or between C1, C2, C3 and A1, A2, A3 or A1, B, D, respectively. Oocytes of better quality (category '1' or '2') were collected at A3 session compared with A1 (P<0.05). Progesterone concentration and oestrous cycle length did not differ among groups. Decreased oestradiol concentrations followed by FSH increase were recorded 3-5. h after OPU. The results confirm the regulatory role of oestradiol on FSH secretion. The quality of collected oocytes was improved in subsequent pick-up sessions in the oestrous cycle. Moreover, OPU at specific stages of the luteal phase of the cycle, even when applied repeatedly, do not affect the normal oestrous cycle length of ewes. © 2013 Elsevier B.V.	en
heal.journalName	Animal Reproduction Science	en
dc.identifier.issue	3-4	en
dc.identifier.volume	138	en
dc.identifier.doi	10.1016/j.anireprosci.2013.02.026	en
dc.identifier.spage	180	en
dc.identifier.epage	187	en