| dc.contributor.author |
Malandrakis, AA |
en |
| dc.contributor.author |
Vattis, KN |
en |
| dc.contributor.author |
Doukas, EG |
en |
| dc.contributor.author |
Markoglou, AN |
en |
| dc.date.accessioned |
2014-06-06T06:52:28Z |
|
| dc.date.available |
2014-06-06T06:52:28Z |
|
| dc.date.issued |
2013 |
en |
| dc.identifier.issn |
01681605 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.ijfoodmicro.2013.05.019 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/6027 |
|
| dc.subject |
Fludioxonil |
en |
| dc.subject |
Fungicide resistance |
en |
| dc.subject |
HPLC |
en |
| dc.subject |
Osmotic stress |
en |
| dc.subject |
OTA |
en |
| dc.subject |
OTB |
en |
| dc.subject.other |
aromatic hydrocarbon |
en |
| dc.subject.other |
chloronitrile chlorothalonil |
en |
| dc.subject.other |
cyprodinil |
en |
| dc.subject.other |
dicarboximide |
en |
| dc.subject.other |
fludioxonil |
en |
| dc.subject.other |
fungicide |
en |
| dc.subject.other |
iprodione |
en |
| dc.subject.other |
mycotoxin |
en |
| dc.subject.other |
ochratoxin |
en |
| dc.subject.other |
pyrrole derivative |
en |
| dc.subject.other |
triazole epoxiconazole |
en |
| dc.subject.other |
unclassified drug |
en |
| dc.subject.other |
vinclozolin |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
Aspergillus carbonarius |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
fungal strain |
en |
| dc.subject.other |
fungus isolation |
en |
| dc.subject.other |
fungus spore |
en |
| dc.subject.other |
grape |
en |
| dc.subject.other |
microbial contamination |
en |
| dc.subject.other |
mutagenesis |
en |
| dc.subject.other |
mycelial growth |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
osmotic stress |
en |
| dc.subject.other |
pathogenicity |
en |
| dc.subject.other |
wild type |
en |
| dc.subject.other |
Fludioxonil |
en |
| dc.subject.other |
Fungicide resistance |
en |
| dc.subject.other |
HPLC |
en |
| dc.subject.other |
Osmotic stress |
en |
| dc.subject.other |
OTA |
en |
| dc.subject.other |
OTB |
en |
| dc.subject.other |
Aspergillus |
en |
| dc.subject.other |
Dioxoles |
en |
| dc.subject.other |
Drug Resistance, Fungal |
en |
| dc.subject.other |
Fungicides, Industrial |
en |
| dc.subject.other |
Mutation |
en |
| dc.subject.other |
Ochratoxins |
en |
| dc.subject.other |
Pyrroles |
en |
| dc.subject.other |
Vitis |
en |
| dc.subject.other |
Aspergillus carbonarius |
en |
| dc.subject.other |
Vitaceae |
en |
| dc.title |
Effect of phenylpyrrole-resistance on fitness parameters and ochratoxin production in Aspergillus carbonarius |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.ijfoodmicro.2013.05.019 |
en |
| heal.publicationDate |
2013 |
en |
| heal.abstract |
The risk of resistance development to fludioxonil and the potential implications of resistance mutations to ochratoxin production in Aspergillus carbonarius were investigated. Mutants of A. carbonarius highly resistant to phenylpyrroles were isolated at a high mutation frequency after N-MNTG-mutagenesis and selection on media containing fludioxonil. A highly reduced sensitivity to fungicides belonging to the same cross-resistance group (AHDs and phenylpyrroles) such as the aromatic hydrocarbon tolclofos-methyl and the dicarboximide fungicides iprodione and vinclozolin was also observed. No cross-resistance relationships were found between fludioxonil and the triazole epoxiconazole, the anilinopyrimidine cyprodinil and the chloronitrile chlorothalonil. Interestingly, fludioxonil-resistant isolates were highly sensitive to the QoI fungicide pyraclostrobin compared to the wild-type parental strain. Fitness studies revealed that resistance mutation(s) had a negative effect on mycelial growth, resistance to osmotic stress and pathogenicity of the fludioxonil-resistant strains. Mycotoxin analysis showed that most fludioxonil-resistant strains produce less quantities of ochratoxin A (OTA) than the wild-type strain both when grown on artificial medium and on grapes. Increased osmotic sensitivity and reduced pathogenicity of the mutant strains were significantly correlated with reduced ochratoxin production in vivo but not in vitro. The above-mentioned data indicate that fludioxonil is an excellent fungicide for the control of A. carbonarius in grapes and a valuable asset for farmers in terms of resistance management and ochratoxin contamination of grapes, vine products and wines. © 2013 Elsevier B.V. |
en |
| heal.journalName |
International Journal of Food Microbiology |
en |
| dc.identifier.issue |
3 |
en |
| dc.identifier.volume |
165 |
en |
| dc.identifier.doi |
10.1016/j.ijfoodmicro.2013.05.019 |
en |
| dc.identifier.spage |
287 |
en |
| dc.identifier.epage |
294 |
en |