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Discrimination of two natural biocontrol agents in the mediterranean region based on mitochondrial DNA sequencing data

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dc.contributor.author Evangelou, VI en
dc.contributor.author Bouga, M en
dc.contributor.author Emmanouel, NG en
dc.contributor.author Perdikis, DC en
dc.contributor.author Papadoulis, GT en
dc.date.accessioned 2014-06-06T06:52:26Z
dc.date.available 2014-06-06T06:52:26Z
dc.date.issued 2013 en
dc.identifier.issn 00062928 en
dc.identifier.uri http://dx.doi.org/10.1007/s10528-013-9610-0 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/6015
dc.subject Genetic variability en
dc.subject Macrolophus spp. en
dc.subject mtDNA en
dc.subject Sequencing en
dc.subject Species discrimination en
dc.subject.other cytochrome c oxidase en
dc.subject.other cytochrome c oxidase subunit 1 en
dc.subject.other RNA 12S en
dc.subject.other unclassified drug en
dc.subject.other article en
dc.subject.other biological control agent en
dc.subject.other biological pest control en
dc.subject.other COI gene en
dc.subject.other DNA sequence en
dc.subject.other female en
dc.subject.other gene amplification en
dc.subject.other genetic variability en
dc.subject.other Greece en
dc.subject.other Hemiptera en
dc.subject.other Macrolophus melanotoma en
dc.subject.other Macrolophus pygmaeus en
dc.subject.other male en
dc.subject.other mitochondrial gene en
dc.subject.other nonhuman en
dc.subject.other nucleotide sequence en
dc.subject.other phylogeny en
dc.subject.other polymerase chain reaction en
dc.subject.other sequence analysis en
dc.subject.other Spain en
dc.subject.other species identification en
dc.subject.other Animals en
dc.subject.other Asteraceae en
dc.subject.other Biological Control Agents en
dc.subject.other Crosses, Genetic en
dc.subject.other DNA, Mitochondrial en
dc.subject.other DNA, Ribosomal en
dc.subject.other Electron Transport Complex IV en
dc.subject.other Female en
dc.subject.other Genes, Mitochondrial en
dc.subject.other Greece en
dc.subject.other Haplotypes en
dc.subject.other Heteroptera en
dc.subject.other Male en
dc.subject.other Mitochondria en
dc.subject.other Phylogeny en
dc.subject.other Sequence Analysis, DNA en
dc.subject.other Solanum en
dc.subject.other Spain en
dc.subject.other Dittrichia viscosa en
dc.subject.other Hemiptera en
dc.subject.other Macrolophus en
dc.subject.other Macrolophus melanotoma en
dc.subject.other Macrolophus pygmaeus en
dc.subject.other Miridae en
dc.subject.other Solanaceae en
dc.title Discrimination of two natural biocontrol agents in the mediterranean region based on mitochondrial DNA sequencing data en
heal.type journalArticle en
heal.identifier.primary 10.1007/s10528-013-9610-0 en
heal.publicationDate 2013 en
heal.abstract Macrolophus pygmaeus and M. melanotoma (Hemiptera: Miridae) are biological control agents used in greenhouse crops, the former preferring plants of the Solanaceae family and the latter the aster Dittrichia viscosa. The discrimination of these species is of high significance for effective biological pest control, but identification based on morphological characters of the host plant is not always reliable. In this study, sequencing analysis of mitochondrial gene segments 12S rDNA and COI has been combined with crossing experiments and morphological observations to develop new markers for Macrolophus spp. discrimination and to provide new data on their genetic variability. This is the first comprehensive research in Greece on M. pygmaeus and M. melanotoma genetic variability based on sequencing data from 12S rDNA and COI gene segments. The relationship of this variability to host plant preference must be investigated in an agricultural ecosystem. © 2013 Springer Science+Business Media New York. en
heal.journalName Biochemical Genetics en
dc.identifier.issue 11-12 en
dc.identifier.volume 51 en
dc.identifier.doi 10.1007/s10528-013-9610-0 en
dc.identifier.spage 825 en
dc.identifier.epage 840 en


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