Differential responsiveness of cortical microtubule orientation to suppression of cell expansion among the developmental zones of Arabidopsis thaliana root apex

dc.contributor.advisor	Panteris, Emmanuel	en
dc.contributor.advisor	Παντερής, Εμμανουήλ	el
dc.contributor.author	Adamakis, Ioannis Dimosthenis S.	en
dc.contributor.author	Daras, Gerasimos	en
dc.contributor.author	Hatzopoulos, Polydefkis	en
dc.contributor.author	Rigas, Stamatis	en
dc.contributor.author	Αδαμάκης, Ιωάννης Δημοσθένης Σ.	el
dc.contributor.author	Δάρας, Γεράσιμος	el
dc.contributor.author	Χατζόπουλος, Πολυδεύκης	el
dc.contributor.author	Ρήγας, Σταμάτης	el
dc.date.accessioned	2014-06-06T06:52:26Z
dc.date.available	2014-06-06T06:52:26Z
dc.date.issued	2013-12-04	en
dc.identifier.issn	19326203	en
dc.identifier.uri	http://dx.doi.org/10.1371/journal.pone.0082442	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/6013
dc.rights	CC0 1.0 Παγκόσμια	el
dc.rights.uri	http://creativecommons.org/publicdomain/zero/1.0/	en
dc.title	Differential responsiveness of cortical microtubule orientation to suppression of cell expansion among the developmental zones of Arabidopsis thaliana root apex	en
heal.type	journalArticle	en
heal.keyword	Cellulose synthase A3	en
heal.keyword	Arabidopsis thaliana	en
heal.keyword	Root development	en
heal.keyword	Cell growth	en
heal.keyword	Cell expansion	en
heal.keyword	Unclassified drug	en
heal.keyword	Binding protein	en
heal.identifier.primary	10.1371/journal.pone.0082442	en
heal.identifier.secondary	e82442	en
heal.recordProvider	Γεωπονικό Πανεπιστήμιο Αθηνών/Γεωπονική Βιοτεχνολογία	el
heal.recordProvider	Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης/Τμήμα Βιολογίας	el
heal.publicationDate	2013-12-04	en
heal.bibliographicCitation	Panteris, Emmanuel. Differential Responsiveness of Cortical Microtubule Orientation to Suppression of Cell Expansion among the Developmental Zones of Arabidopsis thaliana Root Apex, PLoS One, vol. 8 (12), pp 1-17, PLoS 2013	en
heal.abstract	The bidirectional relationship between cortical microtubule orientation and cell wall structure has been extensively studied in elongating cells. Nevertheless, the possible interplay between microtubules and cell wall elements in meristematic cells still remains elusive. Herein, the impact of cellulose synthesis inhibition and suppressed cell elongation on cortical microtubule orientation was assessed throughout the developmental zones of Arabidopsis thaliana root apex by whole-mount tubulin immunolabeling and confocal microscopy. Apart from the wild-type, thanatos and pom2-4 mutants of Cellulose SynthaseA3 and Cellulose Synthase Interacting1 , respectively, were studied. Pharmacological and mechanical approaches inhibiting cell expansion were also applied. Cortical microtubules of untreated wild-type roots were predominantly transverse in the meristematic, transition and elongation root zones. Cellulose-deficient mutants, chemical inhibition of cell expansion, or growth in soil resulted in microtubule reorientation in the elongation zone, wherein cell length was significantly decreased. Combinatorial genetic and chemical suppression of cell expansion extended microtubule reorientation to the transition zone. According to the results, transverse cortical microtubule orientation is established in the meristematic root zone, persisting upon inhibition of cell expansion. Microtubule reorientation in the elongation zone could be attributed to conditional suppression of cell elongation. The differential responsiveness of microtubule orientation to genetic and environmental cues is most likely associated with distinct biophysical traits of the cells among each developmental root zone. © 2013 Panteris et al.	en
heal.publisher	Public Library of Science (PLoS)	en
heal.journalName	PLoS ONE	en
dc.identifier.doi	10.1371/journal.pone.0082442	en