Differential protein expression patterns between planktonic and biofilm cells of Salmonella enterica serovar Enteritidis PT4 on stainless steel surface

dc.contributor.author	Giaouris, E	en
dc.contributor.author	Samoilis, G	en
dc.contributor.author	Chorianopoulos, N	en
dc.contributor.author	Ercolini, D	en
dc.contributor.author	Nychas, G-J	en
dc.date.accessioned	2014-06-06T06:52:26Z
dc.date.available	2014-06-06T06:52:26Z
dc.date.issued	2013	en
dc.identifier.issn	01681605	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.ijfoodmicro.2012.12.023	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/6012
dc.subject	Biofilms	en
dc.subject	Cellular physiology	en
dc.subject	Planktonic	en
dc.subject	Proteomics	en
dc.subject	Salmonella enterica Enteritidis	en
dc.subject	Stainless steel	en
dc.subject.other	aerobic respiration control protein A	en
dc.subject.other	bacterial protein	en
dc.subject.other	BtuE protein	en
dc.subject.other	Crr protein	en
dc.subject.other	CsgF protein	en
dc.subject.other	DppA protein	en
dc.subject.other	Dps protein	en
dc.subject.other	ferric uptake regulator	en
dc.subject.other	GcvT protein	en
dc.subject.other	GpmA protein	en
dc.subject.other	MipA protein	en
dc.subject.other	OsmY protein	en
dc.subject.other	peptidoglycan	en
dc.subject.other	RibB protein	en
dc.subject.other	ssb protein	en
dc.subject.other	SseA protein	en
dc.subject.other	SspA protein	en
dc.subject.other	stainless steel	en
dc.subject.other	SufC protein	en
dc.subject.other	TrxA protein	en
dc.subject.other	unclassified drug	en
dc.subject.other	YbbN protein	en
dc.subject.other	YhbO protein	en
dc.subject.other	YibF protein	en
dc.subject.other	article	en
dc.subject.other	bacterial cell	en
dc.subject.other	biofilm	en
dc.subject.other	controlled study	en
dc.subject.other	detoxification	en
dc.subject.other	DNA metabolism	en
dc.subject.other	energy metabolism	en
dc.subject.other	matrix assisted laser desorption ionization time of flight mass spectrometry	en
dc.subject.other	nonhuman	en
dc.subject.other	plankton	en
dc.subject.other	polyacrylamide gel electrophoresis	en
dc.subject.other	protein analysis	en
dc.subject.other	protein expression	en
dc.subject.other	protein function	en
dc.subject.other	protein synthesis	en
dc.subject.other	regulatory mechanism	en
dc.subject.other	Salmonella enterica	en
dc.subject.other	Salmonella enteritidis	en
dc.subject.other	serotype	en
dc.subject.other	stress	en
dc.subject.other	whole cell	en
dc.subject.other	Biofilms	en
dc.subject.other	Gene Expression Profiling	en
dc.subject.other	Gene Expression Regulation, Bacterial	en
dc.subject.other	Plankton	en
dc.subject.other	Proteome	en
dc.subject.other	Salmonella enteritidis	en
dc.subject.other	Stainless Steel	en
dc.title	Differential protein expression patterns between planktonic and biofilm cells of Salmonella enterica serovar Enteritidis PT4 on stainless steel surface	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.ijfoodmicro.2012.12.023	en
heal.publicationDate	2013	en
heal.abstract	In the present study, the proteome of a strain of S. enterica serovar Enteritidis PT4, grown either as biofilm on stainless steel surface or as free-floating (planktonic) in Brain Heart (BH) broth, was investigated in order to detect the strong differences in whole-cell protein expression patterns between the two growth styles. The proteins extracted from both types of cells were subjected to 2-D PAGE, followed by in-gel tryptic digestion, extraction, subsequent MALDI-TOF mass spectrometry (MS) analysis and finally database searches for protein identification. Using this approach, 30 proteins were identified as differentially expressed between the two growth modes on an ""on-off"" basis, that is, proteins that were detected in one case but not in the other. In particular, 20 and 10 proteins were identified in biofilm and planktonic-grown cells, respectively. The group of proteins whose expression was visible only during biofilm growth included proteins involved in global regulation and stress response (ArcA, BtuE, Dps, OsmY, SspA, TrxA, YbbN and YhbO), nutrient transport (Crr, DppA, Fur and SufC), degradation and energy metabolism (GcvT, GpmA, RibB), detoxification (SseA and YibF), DNA metabolism (SSB), curli production (CsgF), and murein synthesis (MipA). To summarize, this study demonstrates that biofilm growth of S. Enteritidis causes distinct changes in protein expression and offers valuable new data regarding some of the proteins presumably involved in this process. The putative role of these proteins in the maintenance of a biofilm community in Salmonella and other bacteria is discussed. © 2012 Elsevier B.V.	en
heal.journalName	International Journal of Food Microbiology	en
dc.identifier.issue	1	en
dc.identifier.volume	162	en
dc.identifier.doi	10.1016/j.ijfoodmicro.2012.12.023	en
dc.identifier.spage	105	en
dc.identifier.epage	113	en