Development and optimization of an ELISA based method to detect Listeria monocytogenes and Escherichia coli O157 in fresh vegetables

dc.contributor.author	Cavaiuolo, M	en
dc.contributor.author	Paramithiotis, S	en
dc.contributor.author	Drosinos, EH	en
dc.contributor.author	Ferrante, A	en
dc.date.accessioned	2014-06-06T06:52:26Z
dc.date.available	2014-06-06T06:52:26Z
dc.date.issued	2013	en
dc.identifier.issn	17599660	en
dc.identifier.uri	http://dx.doi.org/10.1039/c3ay40893k	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/6006
dc.subject.other	Bacteria isolation	en
dc.subject.other	Contaminated product	en
dc.subject.other	Control measures	en
dc.subject.other	Escherichia coli O157	en
dc.subject.other	Food-borne pathogens	en
dc.subject.other	Fresh vegetables	en
dc.subject.other	Limit of detection	en
dc.subject.other	Listeria monocytogenes	en
dc.subject.other	Antibodies	en
dc.subject.other	Bacteria	en
dc.subject.other	Chemical detection	en
dc.subject.other	Escherichia coli	en
dc.subject.other	Losses	en
dc.subject.other	Optimization	en
dc.subject.other	Pathogens	en
dc.subject.other	Food safety	en
dc.title	Development and optimization of an ELISA based method to detect Listeria monocytogenes and Escherichia coli O157 in fresh vegetables	en
heal.type	journalArticle	en
heal.identifier.primary	10.1039/c3ay40893k	en
heal.publicationDate	2013	en
heal.abstract	Food-borne pathogen contamination of fresh produce represents a crucial problem in terms of food safety and economic losses. To avoid outbreaks and release of contaminated products in the market, food producers must assure that safety and control measures are followed throughout the production chain. Since traditional methods are complex and time consuming, the use of rapid and reliable methods is needed for a reproducible detection of low pathogen levels prior to packaging. To respond to this need, an indirect ELISA assay was developed to detect the presence of Listeria monocytogenes and Escherichia coli O157. Bacteria isolation procedure, antibody working concentration and limit of detection were studied and optimized to verify the presence of the two bacteria on cucumber. Incubation times for antigen (overnight, 4 °C), antibodies (60 minutes, 25 °C) and for substrate reaction (30 min, 25 °C) were selected. Results show that the ELISA method was highly sensitive with a detection limit lower than 103 CFU g-1 and relatively fast because bacteria isolation was achieved from 1 to 7 hours. © 2013 The Royal Society of Chemistry.	en
heal.journalName	Analytical Methods	en
dc.identifier.issue	18	en
dc.identifier.volume	5	en
dc.identifier.doi	10.1039/c3ay40893k	en
dc.identifier.spage	4622	en
dc.identifier.epage	4627	en