Conversions of olive mill wastewater-based media by Saccharomyces cerevisiae through sterile and non-sterile bioprocesses

dc.contributor.author	Sarris, D	en
dc.contributor.author	Giannakis, M	en
dc.contributor.author	Philippoussis, A	en
dc.contributor.author	Komaitis, M	en
dc.contributor.author	Koutinas, AA	en
dc.contributor.author	Papanikolaou, S	en
dc.date.accessioned	2014-06-06T06:52:24Z
dc.date.available	2014-06-06T06:52:24Z
dc.date.issued	2013	en
dc.identifier.issn	02682575	en
dc.identifier.uri	http://dx.doi.org/10.1002/jctb.3931	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5992
dc.subject	Bio-ethanol	en
dc.subject	Olive-mill wastewaters	en
dc.subject	Saccharomyces cerevisiae	en
dc.subject	Waste bioremediation	en
dc.subject.other	Batch bioreactors	en
dc.subject.other	Bio-ethanols	en
dc.subject.other	Biomass productions	en
dc.subject.other	Bioreactor cultures	en
dc.subject.other	Non-sterile condition	en
dc.subject.other	Olive mill wastewaters	en
dc.subject.other	Shake-flask experiments	en
dc.subject.other	Waste bioremediation	en
dc.subject.other	Biochemistry	en
dc.subject.other	Bioconversion	en
dc.subject.other	Bioethanol	en
dc.subject.other	Biomass	en
dc.subject.other	Bioreactors	en
dc.subject.other	Bottles	en
dc.subject.other	Ethanol	en
dc.subject.other	Glucose	en
dc.subject.other	Linoleic acid	en
dc.subject.other	Lipids	en
dc.subject.other	Yeast	en
dc.subject.other	alcohol	en
dc.subject.other	phenol derivative	en
dc.subject.other	alcohol production	en
dc.subject.other	article	en
dc.subject.other	axenic culture	en
dc.subject.other	bacterial growth	en
dc.subject.other	bacterial kinetics	en
dc.subject.other	batch fermentation	en
dc.subject.other	batch reactor	en
dc.subject.other	biogenesis	en
dc.subject.other	biomass	en
dc.subject.other	bioprocess	en
dc.subject.other	biosynthesis	en
dc.subject.other	controlled study	en
dc.subject.other	decolorization	en
dc.subject.other	fatty acid analysis	en
dc.subject.other	nonhuman	en
dc.subject.other	olive mill wastewater	en
dc.subject.other	Saccharomyces cerevisiae	en
dc.subject.other	waste component removal	en
dc.subject.other	waste water	en
dc.subject.other	yeast cell	en
dc.subject.other	Saccharomyces cerevisiae	en
dc.title	Conversions of olive mill wastewater-based media by Saccharomyces cerevisiae through sterile and non-sterile bioprocesses	en
heal.type	journalArticle	en
heal.identifier.primary	10.1002/jctb.3931	en
heal.publicationDate	2013	en
heal.abstract	BACKGROUND: Olive mill wastewaters (OMWs) are an important residue and several methods have been proposed for their treatment. RESULTS: Remarkable decolorization (~63%) and phenol removal (~34% w/w) from OMW was achieved. In glucose-based flask sterile cultures, enrichment with OMWs increased ethanol and biomass production compared with cultures without OMWs added. Flask sterile and un-sterilized cultures demonstrated similar kinetic results. Batch-bioreactor trials performed showed higher ethanol and lower biomass quantities compared with the respective shake-flask experiments, while cultures used under un-sterilized conditions revealed equivalent results to the sterile ones. In non-sterile bioreactor cultures, OMWs addition enhanced biomass production in comparison with culture with no OMWs added, whereas ethanol biosynthesis was not affected. The maximum ethanol quantity achieved was 52 g L-1 (conversion yield per sugar consumed of 0.46 g g-1) in a batch bioreactor non-sterilized trial with OMW-glucose enriched medium used as substrate, that presented initial reducing sugars concentration at ~115 g L-1. Fatty acid analysis of cellular lipids demonstrated that in OMW-based media, cellular lipids containing increased concentrations of oleic and linoleic acid were produced in comparison with cultures with no OMWs added. CONCLUSIONS: S. cerevisiae simultaneously produced bio-ethanol and biomass and detoxified OMWs, under non-sterile conditions. © 2012 Society of Chemical Industry.	en
heal.journalName	Journal of Chemical Technology and Biotechnology	en
dc.identifier.issue	5	en
dc.identifier.volume	88	en
dc.identifier.doi	10.1002/jctb.3931	en
dc.identifier.spage	958	en
dc.identifier.epage	969	en