Colorado potato beetle (Coleoptera) gut transcriptome analysis: Expression of RNA interference-related genes

dc.contributor.author	Swevers, L	en
dc.contributor.author	Huvenne, H	en
dc.contributor.author	Menschaert, G	en
dc.contributor.author	Kontogiannatos, D	en
dc.contributor.author	Kourti, A	en
dc.contributor.author	Pauchet, Y	en
dc.contributor.author	Ffrench-Constant, R	en
dc.contributor.author	Smagghe, G	en
dc.date.accessioned	2014-06-06T06:52:23Z
dc.date.available	2014-06-06T06:52:23Z
dc.date.issued	2013	en
dc.identifier.issn	09621075	en
dc.identifier.uri	http://dx.doi.org/10.1111/imb.12054	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5982
dc.subject	454 sequencing	en
dc.subject	Colorado potato beetle	en
dc.subject	dsRNA uptake	en
dc.subject	gut transcriptome	en
dc.subject	Leptinotarsa decemlineata	en
dc.subject	Manduca sexta	en
dc.subject	microRNA	en
dc.subject	piRNA	en
dc.subject	RNA interference	en
dc.subject	RNAi machinery	en
dc.subject	siRNA	en
dc.subject	tobacco hornworm	en
dc.title	Colorado potato beetle (Coleoptera) gut transcriptome analysis: Expression of RNA interference-related genes	en
heal.type	journalArticle	en
heal.identifier.primary	10.1111/imb.12054	en
heal.publicationDate	2013	en
heal.abstract	In the search for new methods of pest control, the potential of RNA interference (RNAi) is being explored. Because the gut is the first barrier for the uptake of double-stranded (ds)RNA, pyrosequencing of the gut transcriptome is a powerful tool for obtaining the necessary sequences for specific dsRNA-mediated pest control. In the present study, a dataset representing the gut transcriptome of the Colorado potato beetle (CPB; Leptinotarsa decemlineata) was generated and analysed for the presence of RNAi-related genes. Almost all selected genes that were implicated in silencing efficiency at different levels in the RNAi pathway (core machinery, associated intracellular factors, dsRNA uptake, antiviral RNAi, nucleases), which uses different types of small RNA (small interfering RNA, microRNA and piwi-RNA), were expressed in the CPB gut. Although the database is of lower quality, the majority of the RNAi genes are also found to be present in the gut transcriptome of the tobacco hornworm [TH; Manduca sexta (19 out of 35 genes analysed)]. The high quality of the CPB transcriptome database will lay the foundation for future gene expression and functional studies regarding the gut and RNAi. © 2013 Royal Entomological Society.	en
heal.journalName	Insect Molecular Biology	en
dc.identifier.issue	6	en
dc.identifier.volume	22	en
dc.identifier.doi	10.1111/imb.12054	en
dc.identifier.spage	668	en
dc.identifier.epage	684	en