Co-Culture with Listeria monocytogenes within a Dual-Species Biofilm Community Strongly Increases Resistance of Pseudomonas putida to Benzalkonium Chloride

dc.contributor.advisor	Giaouris, Efstathios	en
dc.contributor.advisor	Γκιαούρης, Ευστάθιος	en
dc.contributor.author	Chorianopoulos, Nikos	en
dc.contributor.author	Doulgeraki, Agapi	en
dc.contributor.author	Nychas, Georgios John E.	en
dc.contributor.author	Χωριανόπουλος, Νίκος	el
dc.contributor.author	Νυχάς, Γεώργιος Ιωάννης Ε.	el
dc.contributor.author	Δουγλεράκη, Αγάπη	en
dc.date.accessioned	2014-06-06T06:52:22Z
dc.date.available	2014-06-06T06:52:22Z
dc.date.issued	2013-10-10	en
dc.identifier.issn	19326203	en
dc.identifier.uri	http://dx.doi.org/10.1371/journal.pone.0077276	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5981
dc.rights	CC0 1.0 Παγκόσμια	el
dc.rights.uri	http://creativecommons.org/publicdomain/zero/1.0/	en
dc.title	Co-Culture with Listeria monocytogenes within a Dual-Species Biofilm Community Strongly Increases Resistance of Pseudomonas putida to Benzalkonium Chloride	en
heal.type	journalArticle	en
heal.keyword	Listeria monocytogenes	en
heal.keyword	Antibiotic resistance	en
heal.keyword	Bacterium isolation	en
heal.keyword	Pseudomonas putida	en
heal.keyword	Sessile species	en
heal.keyword	Bacterial growth	en
heal.keyword	Benzalkonium chloride	en
heal.identifier.primary	10.1371/journal.pone.0077276	en
heal.identifier.secondary	e77276	en
heal.recordProvider	Γεωπονικό Πανεπιστήμιο Αθηνών/Επιστήμη Τροφίμων και Διατροφής του Ανθρώπου	el
heal.recordProvider	Πανεπιστήμιο Αιγαίου/Επιστήμη Τροφίμων και Διατροφής Ανθρώπου	el
heal.recordProvider	Ελληνικός Γεωργικός Οργανισμός "Δήμητρα"	el
heal.publicationDate	2013-10-10	en
heal.bibliographicCitation	Giaouris, Efstathios. Co-Culture with Listeria monocytogenes within a Dual-Species Biofilm Community Strongly Increases Resistance of Pseudomonas putida to Benzalkonium Chloride, PLoS One, vol. 8 (10), pp 1-14, PLoS 2013	en
heal.abstract	Biofilm formation is a phenomenon occurring almost wherever microorganisms and surfaces exist in close proximity. This study aimed to evaluate the possible influence of bacterial interactions on the ability of Listeria monocytogenes and Pseudomonas putida to develop a dual-species biofilm community on stainless steel (SS), as well as on the subsequent resistance of their sessile cells to benzalkonium chloride (BC) used in inadequate (sub-lethal) concentration (50 ppm). The possible progressive adaptability of mixed-culture biofilms to BC was also investigated. To accomplish these, 3 strains per species were left to develop mixed-culture biofilms on SS coupons, incubated in daily renewable growth medium for a total period of 10 days, under either mono- or dual-species conditions. Each day, biofilm cells were exposed to disinfection treatment. Results revealed that the simultaneous presence of L. monocytogenes strongly increased the resistance of P. putida biofilm cells to BC, while culture conditions (mono-/dual-species) did not seem to significantly influence the resistance of L. monocytogenes biofilm cells. BC mainly killed L. monocytogenes cells when this was applied against the dual-species sessile community during the whole incubation period, despite the fact that from the 2nd day this community was mainly composed (>90%) of P. putida cells. No obvious adaptation to BC was observed in either L. monocytogenes or P. putida biofilm cells. Pulsed field gel electrophoresis (PFGE) analysis showed that the different strains behaved differently with regard to biofilm formation and antimicrobial resistance. Such knowledge on the physiological behavior of mixed-culture biofilms could provide the information necessary to control their formation.	en
heal.publisher	Public Library of Science (PLoS)	en
heal.journalName	PLoS ONE	en
dc.identifier.doi	10.1371/journal.pone.0077276	en