Characterization of the Biocontrol Activity of Pseudomonas fluorescens Strain X Reveals Novel Genes Regulated by Glucose

dc.contributor.advisor	Kremmydas, Gerasimos F.	en
dc.contributor.advisor	Κρεμμύδας, Γεράσιμος Φ.	el
dc.contributor.author	Tampakaki, Anastasia P.	en
dc.contributor.author	Georgakopoulos, Dimitrios G.	en
dc.contributor.author	Ταμπακάκη, Αναστασία Π.	el
dc.contributor.author	Γεωργακόπουλος, Δημήτριος Γ.	el
dc.date.accessioned	2014-06-06T06:52:22Z
dc.date.available	2014-06-06T06:52:22Z
dc.date.issued	2013-04-15	en
dc.identifier.issn	19326203	en
dc.identifier.uri	http://dx.doi.org/10.1371/journal.pone.0061808	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5979
dc.title	Characterization of the Biocontrol Activity of Pseudomonas fluorescens Strain X Reveals Novel Genes Regulated by Glucose	en
heal.type	journalArticle	en
heal.keyword	Glucose	en
heal.keyword	Biological pest control	en
heal.keyword	Chromosomal localization	en
heal.keyword	Glucose oxidation	en
heal.keyword	Nucleotide sequence	en
heal.keyword	Polymerase chain reaction	en
heal.keyword	Pseudomonas fluorescens	en
heal.keyword	Pseudomonas syringae	en
heal.keyword	Pythium ultimum	en
heal.keyword	GCD gene	en
heal.identifier.primary	10.1371/journal.pone.0061808	en
heal.identifier.secondary	e61808	en
heal.recordProvider	Γεωπονικό Πανεπιστήμιο Αθηνών/Επιστήμη Γεωπονικής Βιοτεχνολογίας	el
heal.publicationDate	2013-04-15	en
heal.bibliographicCitation	Kremmydas, Gerasimos F. Characterization of the Biocontrol Activity of Pseudomonas fluorescens Strain X Reveals Novel Genes Regulated by Glucose, PLoS One, vol. 8 (4), pp 1-11, PLoS 2013	en
heal.abstract	Pseudomonas fluorescens strain X, a bacterial isolate from the rhizosphere of bean seedlings, has the ability to suppress damping-off caused by the oomycete Pythium ultimum. To determine the genes controlling the biocontrol activity of strain X, transposon mutagenesis, sequencing and complementation was performed. Results indicate that, biocontrol ability of this isolate is attributed to gcd gene encoding glucose dehydrogenase, genes encoding its co-enzyme pyrroloquinoline quinone (PQQ), and two genes (sup5 and sup6) which seem to be organized in a putative operon. This operon (named supX) consists of five genes, one of which encodes a non-ribosomal peptide synthase. A unique binding site for a GntR-type transcriptional factor is localized upstream of the supX putative operon. Synteny comparison of the genes in supX revealed that they are common in the genus Pseudomonas, but with a low degree of similarity. supX shows high similarity only to the mangotoxin operon of Ps. syringae pv. syringae UMAF0158. Quantitative real-time PCR analysis indicated that transcription of supX is strongly reduced in the gcd and PQQ-minus mutants of Ps. fluorescens strain X. On the contrary, transcription of supX in the wild type is enhanced by glucose and transcription levels that appear to be higher during the stationary phase. Gcd, which uses PQQ as a cofactor, catalyses the oxidation of glucose to gluconic acid, which controls the activity of the GntR family of transcriptional factors. The genes in the supX putative operon have not been implicated before in the biocontrol of plant pathogens by pseudomonads. They are involved in the biosynthesis of an antimicrobial compound by Ps. fluorescens strain X and their transcription is controlled by glucose, possibly through the activity of a GntR-type transcriptional factor binding upstream of this putative operon. © 2013 Kremmydas et al.	en
heal.publisher	Public Library of Science (PLoS)	en
heal.journalName	PLoS ONE	en
dc.identifier.doi	10.1371/journal.pone.0061808	en