| dc.contributor.author |
Bempelou, ED |
en |
| dc.contributor.author |
Vontas, JG |
en |
| dc.contributor.author |
Liapis, KS |
en |
| dc.contributor.author |
Ziogas, VN |
en |
| dc.date.accessioned |
2014-06-06T06:52:20Z |
|
| dc.date.available |
2014-06-06T06:52:20Z |
|
| dc.date.issued |
2013 |
en |
| dc.identifier.issn |
17913691 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/5966 |
|
| dc.relation.uri |
http://www.scopus.com/inward/record.url?eid=2-s2.0-84881055094&partnerID=40&md5=66d942c0cfc53f640553d39fc6399bd1 |
en |
| dc.subject |
Biodegradation |
en |
| dc.subject |
Diazinon |
en |
| dc.subject |
Epiphytic yeasts |
en |
| dc.subject |
Glutathione-S-transferases |
en |
| dc.subject |
Synergists |
en |
| dc.title |
Biodegradation of diazinon by the epiphytic yeasts Rhodotorula glutinis and Rhodotorula rubra |
en |
| heal.type |
journalArticle |
en |
| heal.publicationDate |
2013 |
en |
| heal.abstract |
The capability of the epiphytic yeasts Rhodotorula glutinis and Rhodotorula rubra to biodegrade diazinon in pure cultures and tomato fruits was investigated. Higher biodegradation rates were observed as the concentration of diazinon and the inoculum of the microorganisms were increased, and the yeasts proved to be more active at 25°C and 15°C. The presence of glucose in the mineral nutrient medium, as an extra source of carbon, delayed the biodegradation by Rh. glutinis, while Rh. rubra biodegrade diazinon faster in the presence of glucose. The detection and quantification of diazinon and its degradation product 2-isopropyo-6-methyl-4-pyrimidinol (IMP) were achieved successfully in the LC-MS/MS chromatographic system. The in vitro enzymatic assays applied suggested that glutathione- S-transferases may be involved in the biodegradation of diazinon, a fact that was further enhanced after the addition of the synergists triphenyl phosphate, diethyl maleate and piperonyl butoxide in the biodegradation trials. The decrease of diazinon residues on tomato fruits confirmed the corresponding on pure cultures, resulting in the suggestion that the yeasts Rh. glutinis and Rh. rubra can possibly be used successfully for the removal or detoxification of diazinon residues ontomatoes. © Benaki Phytopathological Institute. |
en |
| heal.journalName |
Hellenic Plant Protection Journal |
en |
| dc.identifier.issue |
2 |
en |
| dc.identifier.volume |
6 |
en |
| dc.identifier.spage |
69 |
en |
| dc.identifier.epage |
82 |
en |