| heal.abstract |
A strain of the lineage Brachionus 'Nevada' was batch cultured with two diets, differing in biochemical composition: baker's yeast (treatment 1), which has higher protein:lipid ratio compared with CULTURE SELCO (treatment 2). The biochemical composition (DNA, RNA, lipid, protein content) and fatty acid profile of rotifers of both treatments was analysed and related to previously published population structure data. CULTURE SELCO-fed rotifers showed higher DNA, RNA, lipid, n-3 highly unsaturated fatty acids (HUFA) average content, compared with yeast-fed rotifers, which had higher protein content. Rotifer lipid content showed significant diurnal variation in yeast-fed rotifers. Rotifer lipid and n-3 HUFA content was associated with reproductive output. DNA and RNA content was related to embryonic development while protein content, to somatic growth and mixis. The saturated and monounsaturated fatty acid rotifer content was stable irrespective of feed, in contrast to eicosapentanoic acid (EPA) and DHA. The levels of AA were similar in both rotifer populations, but those of EPA and docosahexaenoic acid (DHA) were about half in yeast-fed compared with CULTURE SELCO-fed rotifers. CULTURE SELCO resulted in a temporally stable rotifer lipid profile and a better enriched parthenogenetic population. © 2013 John Wiley & Sons Ltd. |
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