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Antioxidant and enzyme inhibitory activities of blueberry anthocyanins prepared using different solvents

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dc.contributor.author Flores, FP en
dc.contributor.author Singh, RK en
dc.contributor.author Kerr, WL en
dc.contributor.author Pegg, RB en
dc.contributor.author Kong, F en
dc.date.accessioned 2014-06-06T06:52:17Z
dc.date.available 2014-06-06T06:52:17Z
dc.date.issued 2013 en
dc.identifier.issn 00218561 en
dc.identifier.uri http://dx.doi.org/10.1021/jf400429f en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/5950
dc.subject α-glucosidase en
dc.subject anthocyanin en
dc.subject antioxidant en
dc.subject blueberries en
dc.subject solvents en
dc.subject.other Anthocyanin content en
dc.subject.other blueberries en
dc.subject.other Comparison of mass spectrum en
dc.subject.other Different solvents en
dc.subject.other Ferric reducing antioxidant power en
dc.subject.other Glucosidase en
dc.subject.other Inhibitory activity en
dc.subject.other Preparation method en
dc.subject.other Acetone en
dc.subject.other Amylases en
dc.subject.other Antioxidants en
dc.subject.other Bioactivity en
dc.subject.other Mass spectrometry en
dc.subject.other Powders en
dc.subject.other Solvents en
dc.subject.other Anthocyanins en
dc.subject.other acetone en
dc.subject.other alcohol en
dc.subject.other alpha glucosidase en
dc.subject.other amylase en
dc.subject.other anthocyanin en
dc.subject.other antioxidant en
dc.subject.other methanol en
dc.subject.other phenol derivative en
dc.subject.other plant extract en
dc.subject.other solvent en
dc.subject.other article en
dc.subject.other blueberry en
dc.subject.other chemistry en
dc.subject.other colorimetry en
dc.subject.other comparative study en
dc.subject.other drug antagonism en
dc.subject.other food handling en
dc.subject.other freeze drying en
dc.subject.other methodology en
dc.subject.other Acetone en
dc.subject.other alpha-Amylases en
dc.subject.other alpha-Glucosidases en
dc.subject.other Anthocyanins en
dc.subject.other Antioxidants en
dc.subject.other Blueberry Plant en
dc.subject.other Colorimetry en
dc.subject.other Ethanol en
dc.subject.other Food Handling en
dc.subject.other Freeze Drying en
dc.subject.other Methanol en
dc.subject.other Phenols en
dc.subject.other Plant Extracts en
dc.subject.other Solvents en
dc.title Antioxidant and enzyme inhibitory activities of blueberry anthocyanins prepared using different solvents en
heal.type journalArticle en
heal.identifier.primary 10.1021/jf400429f en
heal.publicationDate 2013 en
heal.abstract We compared the biological activities of anthocyanins prepared from whole blueberries or pomace and extracted with acetone, ethanol, and methanol. Crude Amberlite extracts (CAE) and rehydrated powders of freeze-dried anthocyanins were used. Ethanolic CAE yielded the highest total monomeric anthocyanin content [TMAC] (160 ppm), ferric reducing antioxidant power [FRAP] (3.4 mM Fe 2+), total phenolics content [TPC] (382 ppm gallic acid equivalents [GAE]), and α-amylase inhibitory activity (36.8%). The rehydrated powder from acetonic extract gave the greatest FRAP (5.19) and TPC (422.7). α-Amylase (26.1%) and α-glucosidase (91.5%) inhibitory activities were also sustained. Methanolic CAE yielded values intermediate between ethanolic and acetonic extracts. Comparison of mass spectra between Amberlite extracts and rehydrated preparations revealed putative degradation and dimerization products in the rehydrated powders, which could account for loss in biological activities for rehydrated methanolic and ethanolic powders. Results of this study provide useful information in optimizing anthocyanin preparation methods for improved biological activity. © 2013 American Chemical Society. en
heal.journalName Journal of Agricultural and Food Chemistry en
dc.identifier.issue 18 en
dc.identifier.volume 61 en
dc.identifier.doi 10.1021/jf400429f en
dc.identifier.spage 4441 en
dc.identifier.epage 4447 en


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