An in vitro study of Lactobacillus plantarum strains for the presence of plantaricin genes and their potential control of the table olive microbiota

dc.contributor.author	Doulgeraki, AI	en
dc.contributor.author	Paraskevopoulos, N	en
dc.contributor.author	Nychas, GJE	en
dc.contributor.author	Panagou, EZ	en
dc.date.accessioned	2014-06-06T06:52:17Z
dc.date.available	2014-06-06T06:52:17Z
dc.date.issued	2013	en
dc.identifier.issn	00036072	en
dc.identifier.uri	http://dx.doi.org/10.1007/s10482-012-9864-2	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5941
dc.subject	Bacteriocins	en
dc.subject	Lactobacillus plantarum	en
dc.subject	Olive fermentation	en
dc.subject	Real-Time PCR	en
dc.subject.other	bacterial protein	en
dc.subject.other	bacteriocin	en
dc.subject.other	pheromone	en
dc.subject.other	sodium chloride	en
dc.subject.other	agar diffusion	en
dc.subject.other	antimicrobial activity	en
dc.subject.other	article	en
dc.subject.other	Aureobasidium pullulans	en
dc.subject.other	bacterial gene	en
dc.subject.other	bacterial growth	en
dc.subject.other	bacterial strain	en
dc.subject.other	bacterium culture	en
dc.subject.other	bacterium isolation	en
dc.subject.other	controlled study	en
dc.subject.other	culture medium	en
dc.subject.other	fermentation	en
dc.subject.other	gene expression	en
dc.subject.other	gene locus	en
dc.subject.other	genetic regulation	en
dc.subject.other	growth inhibition	en
dc.subject.other	in vitro study	en
dc.subject.other	Lactobacillus pentosus	en
dc.subject.other	Lactobacillus plantarum	en
dc.subject.other	microflora	en
dc.subject.other	nonhuman	en
dc.subject.other	olive	en
dc.subject.other	plantaricin gene	en
dc.subject.other	priority journal	en
dc.subject.other	real time polymerase chain reaction	en
dc.subject.other	salinity	en
dc.subject.other	Ascomycota	en
dc.subject.other	Bacteriocins	en
dc.subject.other	Gene Expression Profiling	en
dc.subject.other	Lactobacillus plantarum	en
dc.subject.other	Olea	en
dc.subject.other	Peptides	en
dc.subject.other	Pheromones	en
dc.subject.other	Real-Time Polymerase Chain Reaction	en
dc.subject.other	Aureobasidium pullulans	en
dc.subject.other	Lactobacillus pentosus	en
dc.subject.other	Lactobacillus plantarum	en
dc.subject.other	Oleaceae	en
dc.title	An in vitro study of Lactobacillus plantarum strains for the presence of plantaricin genes and their potential control of the table olive microbiota	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s10482-012-9864-2	en
heal.publicationDate	2013	en
heal.abstract	Sixteen Lactobacillus plantarum strains, isolated from fermented table olives, were studied for the presence and expression of genes involved in the production of bacteriocins, pheromones and other peptides. The presence of 13 genes that belong to pln locus was monitored, while for the study of gene expression, producer strains were cultured in growth medium with variant salinity (0, 4, 6, and 8 % NaCl) and pH (3.5, 4.0, 4.5, and 6.4). The effect of producer strain on the growth of indicator microorganisms was evaluated using a well diffusion assay. In parallel, Real-Time PCR was employed to monitor the genetic expression of plnE/F and plnJ/K genes for strains that revealed the highest antimicrobial activity. The well diffusion assay showed that the growth of Lactobacillus pentosus was inhibited by six L. plantarum strains when cultured on control medium (0 % NaCl, pH 6.4). Moreover, when the same growth medium was supplemented with 4 and 6 % NaCl, the growth of L. pentosus was inhibited by three and two L. plantarum strains, respectively. Growth of L. pentosus was favoured when L. plantarum strains were cultured on a growth medium with lowered pH (3.5, 4.0, and 4.5). No inhibition of pathogens was observed, but in a few cases, inhibition of Aureobasidium pullulans was detected. The Real-Time PCR assay revealed that the expression of genes was dependent on producer strains and growth phase, whereas inhibition of indicator strains was enhanced in earlier stages of the growth curve in the presence of NaCl, although similar counts were obtained. © 2012 Springer Science+Business Media Dordrecht.	en
heal.journalName	Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology	en
dc.identifier.issue	4	en
dc.identifier.volume	103	en
dc.identifier.doi	10.1007/s10482-012-9864-2	en
dc.identifier.spage	821	en
dc.identifier.epage	832	en