| dc.contributor.author |
Laliotis, GP |
en |
| dc.contributor.author |
Bizelis, I |
en |
| dc.contributor.author |
Vitsa, A |
en |
| dc.contributor.author |
Rogdakis, E |
en |
| dc.date.accessioned |
2014-06-06T06:52:14Z |
|
| dc.date.available |
2014-06-06T06:52:14Z |
|
| dc.date.issued |
2012 |
en |
| dc.identifier.issn |
10495398 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1080/10495398.2011.639423 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/5917 |
|
| dc.subject |
base pair |
en |
| dc.subject |
body weight |
en |
| dc.subject |
bp |
en |
| dc.subject |
BW |
en |
| dc.subject |
cDNA |
en |
| dc.subject |
complementary to RNA |
en |
| dc.subject |
G6PD, glucose 6-phosphate dehydrogenase |
en |
| dc.subject |
ICDH, isocitrate dehydrogenase |
en |
| dc.subject |
NADPH, reduced nicotinamide adenine dinucleotide phosphate |
en |
| dc.subject |
NEL, Net energy lactation |
en |
| dc.subject |
PCR, polymerase chain reaction |
en |
| dc.subject.other |
glucose 6 phosphate dehydrogenase |
en |
| dc.subject.other |
isocitrate dehydrogenase |
en |
| dc.subject.other |
animal |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
caloric intake |
en |
| dc.subject.other |
energy metabolism |
en |
| dc.subject.other |
enzymology |
en |
| dc.subject.other |
female |
en |
| dc.subject.other |
gene expression profiling |
en |
| dc.subject.other |
genetics |
en |
| dc.subject.other |
lactation |
en |
| dc.subject.other |
lipogenesis |
en |
| dc.subject.other |
metabolism |
en |
| dc.subject.other |
physiology |
en |
| dc.subject.other |
polymerase chain reaction |
en |
| dc.subject.other |
sheep |
en |
| dc.subject.other |
subcutaneous fat |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
Energy Intake |
en |
| dc.subject.other |
Energy Metabolism |
en |
| dc.subject.other |
Female |
en |
| dc.subject.other |
Gene Expression Profiling |
en |
| dc.subject.other |
Glucosephosphate Dehydrogenase |
en |
| dc.subject.other |
Isocitrate Dehydrogenase |
en |
| dc.subject.other |
Lactation |
en |
| dc.subject.other |
Lipogenesis |
en |
| dc.subject.other |
Polymerase Chain Reaction |
en |
| dc.subject.other |
Sheep, Domestic |
en |
| dc.subject.other |
Subcutaneous Fat |
en |
| dc.subject.other |
Ovis |
en |
| dc.title |
Increase of energy balance significantly alters major lipogenic gene expression in lactation ewes |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1080/10495398.2011.639423 |
en |
| heal.publicationDate |
2012 |
en |
| heal.abstract |
The objective of the present study was to examine changes observed in the expression of cytosolic NADP isocitrate dehydrogenase (ICDH) and glucose 6-phosphate dehydrogenase (G6PD) genes, the major implicated genes in ruminant lipogenesis in terms of produce NADPH, during the early post-weaning period in dairy ewes in respect to energy intake, and to further correlate the noted changes with their respective enzymatic activities. A total of 21 subcutaneous adipose tissue samples were obtained from seven lactating (2nd lactation period) dairy ewes of the Chios breed. Adipose tissue samples were taken from the tail head region at weeks 1, 2, and 4 after weaning (45 days after parturition). Dairy ewes were in negative energy balance during weeks 1 and 2 after weaning and they moved into a strong positive energy balance at week 4 after weaning. Expression of ICDH and G6PD genes and their respective enzymatic activity was determined. Results showed that both genes expression and enzymatic activities were significantly minimal at week 1 after weaning, reaching a maximum level at week 4 after weaning (P<0.05). A 3.5-fold and a 5-fold increase of G6PD and ICDH mRNA levels were observed, respectively. Concerning their respective enzymatic activities, a 5.5-fold and 2-fold increase was noted, respectively. A positive correlation was found between ICDH and G6PD gene expression (P<0.001) indicating a synchronized response to energy intake changes. Almost similar changes were observed for enzymatic activities, rendering these enzymes as potential biochemical markers of ovine lipogenesis © 2012 Copyright Taylor and Francis Group, LLC. |
en |
| heal.journalName |
Animal Biotechnology |
en |
| dc.identifier.issue |
1 |
en |
| dc.identifier.volume |
23 |
en |
| dc.identifier.doi |
10.1080/10495398.2011.639423 |
en |
| dc.identifier.spage |
64 |
en |
| dc.identifier.epage |
69 |
en |