Triiodothyronine stimulates glucose transport in bone cells

dc.contributor.author	Zoidis, E	en
dc.contributor.author	Ghirlanda-Keller, C	en
dc.contributor.author	Schmid, C	en
dc.date.accessioned	2014-06-06T06:52:11Z
dc.date.available	2014-06-06T06:52:11Z
dc.date.issued	2012	en
dc.identifier.issn	1355008X	en
dc.identifier.uri	http://dx.doi.org/10.1007/s12020-012-9594-2	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5888
dc.subject	Glucose transport	en
dc.subject	GLUT	en
dc.subject	Osteoblast	en
dc.subject	Triiodothyronine	en
dc.subject.other	2,4 dinitrophenol	en
dc.subject.other	alkaline phosphatase bone isoenzyme	en
dc.subject.other	carbon 14	en
dc.subject.other	deoxyglucose	en
dc.subject.other	glucose	en
dc.subject.other	glucose transporter 1	en
dc.subject.other	glucose transporter 3	en
dc.subject.other	liothyronine	en
dc.subject.other	messenger RNA	en
dc.subject.other	parathyroid hormone	en
dc.subject.other	protein	en
dc.subject.other	somatomedin C	en
dc.subject.other	animal cell	en
dc.subject.other	article	en
dc.subject.other	bone densitometry	en
dc.subject.other	cell count	en
dc.subject.other	cell membrane	en
dc.subject.other	concentration response	en
dc.subject.other	controlled study	en
dc.subject.other	culture medium	en
dc.subject.other	energy metabolism	en
dc.subject.other	gene expression	en
dc.subject.other	glucose transport	en
dc.subject.other	hormone action	en
dc.subject.other	newborn	en
dc.subject.other	nonhuman	en
dc.subject.other	Northern blotting	en
dc.subject.other	nucleotide sequence	en
dc.subject.other	osteoblast	en
dc.subject.other	priority journal	en
dc.subject.other	protein content	en
dc.subject.other	protein expression	en
dc.subject.other	rat	en
dc.subject.other	Western blotting	en
dc.subject.other	Animals	en
dc.subject.other	Biological Transport	en
dc.subject.other	Calcitriol	en
dc.subject.other	Cell Line	en
dc.subject.other	Cell Membrane	en
dc.subject.other	Culture Media, Serum-Free	en
dc.subject.other	Deoxyglucose	en
dc.subject.other	Glucose	en
dc.subject.other	Glucose Transporter Type 1	en
dc.subject.other	Glucose Transporter Type 3	en
dc.subject.other	Insulin-Like Growth Factor I	en
dc.subject.other	Kinetics	en
dc.subject.other	Osteoblasts	en
dc.subject.other	Parathyroid Hormone	en
dc.subject.other	Peptide Fragments	en
dc.subject.other	Protein Biosynthesis	en
dc.subject.other	Rats	en
dc.subject.other	Recombinant Proteins	en
dc.subject.other	RNA, Messenger	en
dc.subject.other	Triiodothyronine	en
dc.subject.other	Up-Regulation	en
dc.title	Triiodothyronine stimulates glucose transport in bone cells	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s12020-012-9594-2	en
heal.publicationDate	2012	en
heal.abstract	Thyroid hormones increase energy expenditure and bone turnover in vivo. To study whether 3,3′,5-triiodol-thyronine (T3) stimulates the uptake of glucose in osteoblastic cells, PyMS (a cell line derived from rat bone) cells were kept in serum-free culture medium and treated with T 3. We measured [1-14C]-2-deoxy-D-glucose (2DG) uptake and looked for expression of the high-affinity glucose transporters GLUT1 and GLUT3 by northern and western analysis. T3 did not influence the cell number but slightly (1.3-fold) increased the protein content of the cell cultures. 2DG uptake was low in serum-deprived cell cultures and was increased by T3 (up to 2.5-fold at 1 nmol l-1 after 4 days) in a dose- and time-dependent manner. Triiodothyronine at 1 nmol l-1 increased GLUT1 and GLUT3 abundance in membranes. Therefore, increased glucose uptake induced by T3 in osteoblasts may be mediated by the known high-affinity glucose transporters GLUT1 and GLUT3. © Springer Science+Business Media, LLC 2012.	en
heal.journalName	Endocrine	en
dc.identifier.issue	3	en
dc.identifier.volume	41	en
dc.identifier.doi	10.1007/s12020-012-9594-2	en
dc.identifier.spage	501	en
dc.identifier.epage	511	en