The Caenorhabditis elegans parvulin gene subfamily and their expression under cold or heat stress along with the fkb subfamily

dc.contributor.author	Fasseas, MK	en
dc.contributor.author	Dimou, M	en
dc.contributor.author	Katinakis, P	en
dc.date.accessioned	2014-06-06T06:52:08Z
dc.date.available	2014-06-06T06:52:08Z
dc.date.issued	2012	en
dc.identifier.issn	0006291X	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.bbrc.2012.05.157	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5860
dc.subject	C. elegans	en
dc.subject	Cold	en
dc.subject	Fkb	en
dc.subject	Heat	en
dc.subject	Parvulin	en
dc.subject	Stress	en
dc.subject.other	double stranded RNA	en
dc.subject.other	fk 506 binding protein	en
dc.subject.other	article	en
dc.subject.other	Caenorhabditis elegans	en
dc.subject.other	cold stress	en
dc.subject.other	computer model	en
dc.subject.other	controlled study	en
dc.subject.other	enzyme activity	en
dc.subject.other	Escherichia coli	en
dc.subject.other	fkb gene	en
dc.subject.other	gene	en
dc.subject.other	gene expression	en
dc.subject.other	heat stress	en
dc.subject.other	lifespan	en
dc.subject.other	multigene family	en
dc.subject.other	mutant	en
dc.subject.other	nematode life cycle stage	en
dc.subject.other	nonhuman	en
dc.subject.other	parvulin gene	en
dc.subject.other	pin 4 gene	en
dc.subject.other	priority journal	en
dc.subject.other	real time polymerase chain reaction	en
dc.subject.other	reverse transcription polymerase chain reaction	en
dc.subject.other	RNA interference	en
dc.subject.other	wild type	en
dc.subject.other	Animals	en
dc.subject.other	Caenorhabditis elegans	en
dc.subject.other	Caenorhabditis elegans Proteins	en
dc.subject.other	Cold-Shock Response	en
dc.subject.other	Escherichia coli	en
dc.subject.other	Gene Expression Regulation, Enzymologic	en
dc.subject.other	Heat-Shock Response	en
dc.subject.other	Peptidylprolyl Isomerase	en
dc.subject.other	Real-Time Polymerase Chain Reaction	en
dc.subject.other	RNA Interference	en
dc.subject.other	Caenorhabditis elegans	en
dc.subject.other	Escherichia coli	en
dc.title	The Caenorhabditis elegans parvulin gene subfamily and their expression under cold or heat stress along with the fkb subfamily	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.bbrc.2012.05.157	en
heal.publicationDate	2012	en
heal.abstract	Parvulins and FKBPs are members of the peptidyl-prolyl cis/trans isomerases (PPIase) enzyme family whose role is to catalyze the interconversion between the cis trans forms of a peptide bond preceding internal proline residues in a polypeptide substrate. Members of the parvulin subfamily have been found to be involved in a variety of diseases, including Alzheimer's disease and cancer and are also considered possible antiparasitic targets. Genes Y110A2AL.13 (pin-1) and Y48C3A.16 (pin-4) were found in the worm's genome, possibly encoding parvulins. One is homologous to human and fly PIN1 whereas the other is homologous to human and fly PIN4. Both were expressed in Escherichia coli, purified and found to have in vitro PPIase activity. Expression levels of both genes, as well as the fkb genes (that encode FK506-binding proteins) were measured during development and under cold or heat stress conditions. The results revealed a potential role for these genes under temperature-related stress. RNAi silencing was performed for wild type and mutant strain worms under normal and cold or heat stress conditions. A reduced lifespan was observed when pin-4 dsRNA was fed to the fkb-5 deficient worms. Our work presents a first attempt to characterize the Caenorhabditis elegans parvulins and may present an interesting starting point for further experimentation concerning their role, along with the FKBP subfamily, in nematode physiology and their possible use as antiparasitic targets. © 2012 Elsevier Inc.	en
heal.journalName	Biochemical and Biophysical Research Communications	en
dc.identifier.issue	3	en
dc.identifier.volume	423	en
dc.identifier.doi	10.1016/j.bbrc.2012.05.157	en
dc.identifier.spage	520	en
dc.identifier.epage	525	en