Terpenes transfer to milk and cheese after oral administration to sheep fed indoors

dc.contributor.author	Poulopoulou, I	en
dc.contributor.author	Zoidis, E	en
dc.contributor.author	Massouras, T	en
dc.contributor.author	Hadjigeorgiou, I	en
dc.date.accessioned	2014-06-06T06:52:08Z
dc.date.available	2014-06-06T06:52:08Z
dc.date.issued	2012	en
dc.identifier.issn	09312439	en
dc.identifier.uri	http://dx.doi.org/10.1111/j.1439-0396.2011.01128.x	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5857
dc.subject	Animal products	en
dc.subject	Blood	en
dc.subject	Feed tracers	en
dc.subject	Milk	en
dc.subject	Sheep	en
dc.subject	Terpenes	en
dc.subject.other	mineral	en
dc.subject.other	terpene	en
dc.subject.other	animal	en
dc.subject.other	animal food	en
dc.subject.other	article	en
dc.subject.other	blood	en
dc.subject.other	cheese	en
dc.subject.other	chemistry	en
dc.subject.other	female	en
dc.subject.other	metabolism	en
dc.subject.other	milk	en
dc.subject.other	sheep	en
dc.subject.other	time	en
dc.subject.other	Animal Feed	en
dc.subject.other	Animals	en
dc.subject.other	Cheese	en
dc.subject.other	Female	en
dc.subject.other	Milk	en
dc.subject.other	Minerals	en
dc.subject.other	Sheep	en
dc.subject.other	Terpenes	en
dc.subject.other	Time Factors	en
dc.subject.other	Animalia	en
dc.subject.other	Ovis aries	en
dc.title	Terpenes transfer to milk and cheese after oral administration to sheep fed indoors	en
heal.type	journalArticle	en
heal.identifier.primary	10.1111/j.1439-0396.2011.01128.x	en
heal.publicationDate	2012	en
heal.abstract	Terpenes have been proposed as potential biomarkers in verifying the diets of grazing animals. A study of the relationships between the intake of terpenes and their presence in animal tissues (blood and milk) as well as in the final product (cheese) was conducted. Eight dairy sheep were divided into two equal groups, representing control (C) and treatment group (T). In T group oral administration of a mixture of terpenes, α-pinene, limonene and β-caryophyllene, was applied over a period of 18days. Blood and milk samples were collected regularly and terpenes were identified by extraction using petroleum ether and the solid phase micro-extraction (SPME) method, respectively, followed by GC-MS analysis. Cheese was produced, from C and T animals separately, twice during the period of terpenes oral administration. Terpenes contents and chemical properties of the produced cheeses were investigated. Limonene and α-pinene were found in all blood and milk samples of the T group after a lag-phase of 2days, while β-caryophyllene was detected in few plasma samples and in all milk samples. None of the terpenes was traced in blood and milk of C animals. The contents of cheese, in dosed terpenes, presented a more complicated pattern suggesting terpenes non-credible as biomarkers. We conclude terpenes can be used as biomarkers for authentification of ewes' milk, but further research is required on factors affecting their transfer to dairy products from grazing diets. © 2011 Blackwell Verlag GmbH.	en
heal.journalName	Journal of Animal Physiology and Animal Nutrition	en
dc.identifier.issue	2	en
dc.identifier.volume	96	en
dc.identifier.doi	10.1111/j.1439-0396.2011.01128.x	en
dc.identifier.spage	172	en
dc.identifier.epage	181	en