| dc.contributor.author |
Rantsiou, K |
en |
| dc.contributor.author |
Greppi, A |
en |
| dc.contributor.author |
Garosi, M |
en |
| dc.contributor.author |
Acquadro, A |
en |
| dc.contributor.author |
Mataragas, M |
en |
| dc.contributor.author |
Cocolin, L |
en |
| dc.date.accessioned |
2014-06-06T06:52:06Z |
|
| dc.date.available |
2014-06-06T06:52:06Z |
|
| dc.date.issued |
2012 |
en |
| dc.identifier.issn |
01681605 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.ijfoodmicro.2011.08.009 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/5841 |
|
| dc.subject |
In situ |
en |
| dc.subject |
Listeria monocytogenes |
en |
| dc.subject |
Microarray |
en |
| dc.subject |
Virulence |
en |
| dc.subject.other |
bacterial protein |
en |
| dc.subject.other |
bacterial RNA |
en |
| dc.subject.other |
bacterial virulence protein |
en |
| dc.subject.other |
gadC protein |
en |
| dc.subject.other |
gadE protein |
en |
| dc.subject.other |
heat shock protein |
en |
| dc.subject.other |
iap protein |
en |
| dc.subject.other |
unclassified drug |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
bacterial gene |
en |
| dc.subject.other |
bacterial strain |
en |
| dc.subject.other |
bacterial virulence |
en |
| dc.subject.other |
bacterium adherence |
en |
| dc.subject.other |
cluster analysis |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
gene expression |
en |
| dc.subject.other |
Listeria monocytogenes |
en |
| dc.subject.other |
meat |
en |
| dc.subject.other |
microarray analysis |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
stress |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
Gene Expression Profiling |
en |
| dc.subject.other |
Genes, Bacterial |
en |
| dc.subject.other |
Listeria monocytogenes |
en |
| dc.subject.other |
Meat |
en |
| dc.subject.other |
Meat Products |
en |
| dc.subject.other |
Oligonucleotide Array Sequence Analysis |
en |
| dc.subject.other |
Stress, Physiological |
en |
| dc.subject.other |
Virulence Factors |
en |
| dc.subject.other |
Listeria monocytogenes |
en |
| dc.title |
Strain dependent expression of stress response and virulence genes of Listeria monocytogenes in meat juices as determined by microarray |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.ijfoodmicro.2011.08.009 |
en |
| heal.publicationDate |
2012 |
en |
| heal.abstract |
A subgenomic array, encompassing 54 probes targeting genes responsible for virulence, adhesion and stress response in Listeria monocytogenes, was used in order to study their expression in food systems. RNA extracted from L. monocytogenes inoculated in BHI and in situ (i.e. in minced meat and fermented sausage juices) and incubated at 4 °C, was hybridized on the array and the results obtained were compared in order to understand the effect that the food juice has on the expression. Three different strains of L. monocytogenes were tested, in order to determine the effect of the strain provenience. As determined by cluster analysis, each strain behaved in a different way when inoculated in food juices. The goal was to respond to acidic and osmotic stresses encountered in the food, particularly in the fermented sausage juice. No differences in the expression profile between the three strains were observed, when they were inoculated in BHI. On the other hand, in the meat and sausage juices, the iap, gadC and gadE genes, together with different internalin encoding genes, were significantly differentially expressed in the three strains. © 2011 Elsevier B.V. |
en |
| heal.journalName |
International Journal of Food Microbiology |
en |
| dc.identifier.issue |
3 |
en |
| dc.identifier.volume |
152 |
en |
| dc.identifier.doi |
10.1016/j.ijfoodmicro.2011.08.009 |
en |
| dc.identifier.spage |
116 |
en |
| dc.identifier.epage |
122 |
en |