| dc.contributor.author |
Politis, I |
en |
| dc.contributor.author |
Theodorou, G |
en |
| dc.contributor.author |
Lampidonis, AD |
en |
| dc.contributor.author |
Chronopoulou, R |
en |
| dc.contributor.author |
Baldi, A |
en |
| dc.date.accessioned |
2014-06-06T06:52:05Z |
|
| dc.date.available |
2014-06-06T06:52:05Z |
|
| dc.date.issued |
2012 |
en |
| dc.identifier.issn |
00071145 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1017/S0007114511006520 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/5837 |
|
| dc.subject |
Fatty acids |
en |
| dc.subject |
Inflammation |
en |
| dc.subject |
Phagocytes |
en |
| dc.subject |
Soya protein hydrolysates |
en |
| dc.subject.other |
cyclooxygenase 2 |
en |
| dc.subject.other |
fatty acid |
en |
| dc.subject.other |
glycoprotein p 15095 |
en |
| dc.subject.other |
inducible nitric oxide synthase |
en |
| dc.subject.other |
intercellular adhesion molecule 1 |
en |
| dc.subject.other |
intercellular adhesion molecule 2 |
en |
| dc.subject.other |
interleukin 10 |
en |
| dc.subject.other |
myristic acid |
en |
| dc.subject.other |
oleic acid |
en |
| dc.subject.other |
palmitic acid |
en |
| dc.subject.other |
palmitoleic acid |
en |
| dc.subject.other |
plasminogen activator inhibitor 1 |
en |
| dc.subject.other |
plasminogen activator inhibitor 2 |
en |
| dc.subject.other |
protein hydrolysate |
en |
| dc.subject.other |
soybean protein |
en |
| dc.subject.other |
stearic acid |
en |
| dc.subject.other |
urokinase |
en |
| dc.subject.other |
urokinase receptor |
en |
| dc.subject.other |
animal cell |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
binding site |
en |
| dc.subject.other |
CD11c gene |
en |
| dc.subject.other |
cell activation |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
cyclooxygenase 2 gene |
en |
| dc.subject.other |
enzyme activity |
en |
| dc.subject.other |
female |
en |
| dc.subject.other |
gene expression |
en |
| dc.subject.other |
in vitro study |
en |
| dc.subject.other |
inducible nitric oxide synthase gene |
en |
| dc.subject.other |
intercellular adhesion molecule 1 gene |
en |
| dc.subject.other |
intercellular adhesion molecule 2 gene |
en |
| dc.subject.other |
interleukin 10 gene |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
phagocyte |
en |
| dc.subject.other |
plasminogen activator inhibitor 1 gene |
en |
| dc.subject.other |
plasminogen activator inhibitor 2 gene |
en |
| dc.subject.other |
urokinase gene |
en |
| dc.subject.other |
urokinase receptor gene |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
Animals, Inbred Strains |
en |
| dc.subject.other |
Cells, Cultured |
en |
| dc.subject.other |
Crosses, Genetic |
en |
| dc.subject.other |
Fatty Acids, Nonesterified |
en |
| dc.subject.other |
Female |
en |
| dc.subject.other |
Functional Food |
en |
| dc.subject.other |
Gene Expression Regulation |
en |
| dc.subject.other |
Immunomodulation |
en |
| dc.subject.other |
Inflammation Mediators |
en |
| dc.subject.other |
Intercellular Adhesion Molecule-1 |
en |
| dc.subject.other |
Macrophage Activation |
en |
| dc.subject.other |
Neutrophil Activation |
en |
| dc.subject.other |
Nitric Oxide Synthase Type II |
en |
| dc.subject.other |
Phagocytes |
en |
| dc.subject.other |
Protein Hydrolysates |
en |
| dc.subject.other |
Receptors, Urokinase Plasminogen Activator |
en |
| dc.subject.other |
RNA, Messenger |
en |
| dc.subject.other |
Sheep, Domestic |
en |
| dc.subject.other |
Soy Foods |
en |
| dc.subject.other |
Soybean Proteins |
en |
| dc.subject.other |
Urokinase-Type Plasminogen Activator |
en |
| dc.subject.other |
Ovis |
en |
| dc.title |
Soya protein hydrolysates modify the expression of various pro-inflammatory genes induced by fatty acids in ovine phagocytes |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1017/S0007114511006520 |
en |
| heal.publicationDate |
2012 |
en |
| heal.abstract |
The objective of the present study was to test the hypothesis that fatty acids are the circulating mediators acting in a pro-inflammatory manner towards activated circulating ovine monocyte/macrophages and neutrophils. Furthermore, whether soya protein hydrolysates (SPH) inhibit the fatty acid-induced increase in the production of pro-inflammatory responses by ovine phagocytes was tested in vitro. All the fatty acids tested (myristic, palmitic, palmitoleic, stearic and oleic) increased (P < 0•01; C18>C16> C14) membrane-bound urokinase plasminogen activator (u-PA) and u-PA free binding sites in cell membranes of activated ovine blood monocytes/macrophages, but only the C18 fatty acids (stearic, oleic) were effective towards blood neutrophils. The C18 fatty acids up-regulated (P < 0•05) the gene expression of u-PA, u-PA receptor, intercellular adhesion molecule 1 and inducible NO synthase (in monocytes) but not that of cyclo-oxygenase-2, integrin α X and plasminogen activator inhibitor types 1 and 2 by ovine phagocytes. SPH blocked completely or partially all C18 fatty acid-induced changes in the expression of various pro-inflammatory genes. In conclusion, fatty acids selectively ""activate"" ovine phagocytes, suggesting that these cells ""sense"" metabolic signals derived from adipocytes. Soya protein peptides inhibit all changes in gene expression induced by fatty acids in ovine phagocytes in vitro. This constitutes a novel mechanism of action. © 2011 The Authors. |
en |
| heal.journalName |
British Journal of Nutrition |
en |
| dc.identifier.issue |
7 |
en |
| dc.identifier.volume |
108 |
en |
| dc.identifier.doi |
10.1017/S0007114511006520 |
en |
| dc.identifier.spage |
1246 |
en |
| dc.identifier.epage |
1255 |
en |