Screening of bacterial strains capable of converting biodiesel-derived raw glycerol into 1,3-propanediol, 2,3-butanediol and ethanol

dc.contributor.author	Metsoviti, M	en
dc.contributor.author	Paramithiotis, S	en
dc.contributor.author	Drosinos, EH	en
dc.contributor.author	Galiotou-Panayotou, M	en
dc.contributor.author	Nychas, G-JE	en
dc.contributor.author	Zeng, A-P	en
dc.contributor.author	Papanikolaou, S	en
dc.date.accessioned	2014-06-06T06:52:03Z
dc.date.available	2014-06-06T06:52:03Z
dc.date.issued	2012	en
dc.identifier.issn	16180240	en
dc.identifier.uri	http://dx.doi.org/10.1002/elsc.201100058	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5827
dc.subject	1,3-Propanediol	en
dc.subject	2,3-Butanediol	en
dc.subject	Biodiesel	en
dc.subject	Ethanol	en
dc.subject	Raw glycerol	en
dc.subject.other	1 ,3 propanediol	en
dc.subject.other	2 ,3-butanediol	en
dc.subject.other	Aerobic condition	en
dc.subject.other	Aerobiosis	en
dc.subject.other	Anaerobic conditions	en
dc.subject.other	Bacterial strains	en
dc.subject.other	Biodiesel facility	en
dc.subject.other	Carbon source	en
dc.subject.other	Clostridium butyricum	en
dc.subject.other	Conversion yield	en
dc.subject.other	Enterobacter aerogenes	en
dc.subject.other	Experimental conditions	en
dc.subject.other	Glycerol concentration	en
dc.subject.other	Klebsiella oxytoca	en
dc.subject.other	Raw glycerol	en
dc.subject.other	Shake-flask experiments	en
dc.subject.other	Volumetric productivity	en
dc.subject.other	Biodiesel	en
dc.subject.other	Bioreactors	en
dc.subject.other	Chemical compounds	en
dc.subject.other	Chemical industry	en
dc.subject.other	Chlorine	en
dc.subject.other	Concentration (process)	en
dc.subject.other	Ethanol	en
dc.subject.other	Metabolism	en
dc.subject.other	Textile industry	en
dc.subject.other	Glycerol	en
dc.subject.other	1,3 propanediol	en
dc.subject.other	2,3 butanediol	en
dc.subject.other	alcohol	en
dc.subject.other	biodiesel	en
dc.subject.other	glycerol	en
dc.subject.other	alcohol	en
dc.subject.other	alternative fuel	en
dc.subject.other	anoxic conditions	en
dc.subject.other	bacterium	en
dc.subject.other	biofuel	en
dc.subject.other	bioreactor	en
dc.subject.other	biotechnology	en
dc.subject.other	biotransformation	en
dc.subject.other	concentration (composition)	en
dc.subject.other	industrial production	en
dc.subject.other	metabolism	en
dc.subject.other	microbial activity	en
dc.subject.other	oxic conditions	en
dc.subject.other	pH	en
dc.subject.other	substrate	en
dc.subject.other	aerobic metabolism	en
dc.subject.other	anaerobic bacterium	en
dc.subject.other	article	en
dc.subject.other	Bacillus licheniformis	en
dc.subject.other	Bacillus subtilis	en
dc.subject.other	bacterial strain	en
dc.subject.other	bacterium culture	en
dc.subject.other	bacterium identification	en
dc.subject.other	bacterium isolation	en
dc.subject.other	bioreactor	en
dc.subject.other	biotransformation	en
dc.subject.other	carbon source	en
dc.subject.other	chemical analysis	en
dc.subject.other	chemical composition	en
dc.subject.other	Citrobacter freundii	en
dc.subject.other	Clostridium butyricum	en
dc.subject.other	concentration (parameters)	en
dc.subject.other	Enterobacter aerogenes	en
dc.subject.other	fermentation	en
dc.subject.other	high performance liquid chromatography	en
dc.subject.other	Klebsiella oxytoca	en
dc.subject.other	Lactobacillus brevis	en
dc.subject.other	nonhuman	en
dc.subject.other	pH	en
dc.subject.other	screening	en
dc.subject.other	yeast	en
dc.subject.other	Bacteria (microorganisms)	en
dc.subject.other	Citrobacter freundii	en
dc.subject.other	Clostridium butyricum	en
dc.subject.other	Enterobacter aerogenes	en
dc.subject.other	Klebsiella oxytoca	en
dc.title	Screening of bacterial strains capable of converting biodiesel-derived raw glycerol into 1,3-propanediol, 2,3-butanediol and ethanol	en
heal.type	journalArticle	en
heal.identifier.primary	10.1002/elsc.201100058	en
heal.publicationDate	2012	en
heal.abstract	The ability of bacterial strains to assimilate glycerol derived from biodiesel facilities to produce metabolic compounds of importance for the food, textile and chemical industry, such as 1,3-propanediol (PD), 2,3-butanediol (BD) and ethanol (EtOH), was assessed. The screening of 84 bacterial strains was performed using glycerol as carbon source. After initial trials, 12 strains were identified capable of consuming raw glycerol under anaerobic conditions, whereas 5 strains consumed glycerol under aerobiosis. A plethora of metabolic compounds was synthesized; in anaerobic batch-bioreactor cultures PD in quantities up to 11.3g/L was produced by Clostridium butyricum NRRL B-23495, while the respective value was 10.1g/L for a newly isolated Citrobacter freundii. Adaptation of Cl. butyricum at higher initial glycerol concentration resulted in a PD max concentration of ~32g/L. BD was produced by a new Enterobacter aerogenes isolate in shake-flask experiments, under fully aerobic conditions, with a maximum concentration of ~22g/L which was achieved at an initial glycerol quantity of 55g/L. A new Klebsiella oxytoca isolate converted waste glycerol into mixtures of PD, BD and EtOH at various ratios. Finally, another new C. freundii isolate converted waste glycerol into EtOH in anaerobic batch-bioreactor cultures with constant pH, achieving a final EtOH concentration of 14.5g/L, a conversion yield of 0.45g/g and a volumetric productivity of ~0.7g/L/h. As a conclusion, the current study confirmed the utilization of biodiesel-derived raw glycerol as an appropriate substrate for the production of PD, BD and EtOH by several newly isolated bacterial strains under different experimental conditions. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.	en
heal.journalName	Engineering in Life Sciences	en
dc.identifier.issue	1	en
dc.identifier.volume	12	en
dc.identifier.doi	10.1002/elsc.201100058	en
dc.identifier.spage	57	en
dc.identifier.epage	68	en