Rapid strain classification and taxa delimitation within the edible mushroom genus Pleurotus through the use of diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy

dc.contributor.author	Zervakis, GI	en
dc.contributor.author	Bekiaris, G	en
dc.contributor.author	Tarantilis, PA	en
dc.contributor.author	Pappas, CS	en
dc.date.accessioned	2014-06-06T06:52:01Z
dc.date.available	2014-06-06T06:52:01Z
dc.date.issued	2012	en
dc.identifier.issn	18786146	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.funbio.2012.04.006	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5812
dc.subject	Filamentous fungi	en
dc.subject	FT-IR spectroscopy	en
dc.subject	Mushroom identification	en
dc.subject	Oyster mushroom	en
dc.subject	Pleurotus taxonomy	en
dc.subject.other	bacterium	en
dc.subject.other	cluster analysis	en
dc.subject.other	cost-benefit analysis	en
dc.subject.other	FTIR spectroscopy	en
dc.subject.other	mushroom	en
dc.subject.other	substrate	en
dc.subject.other	taxonomy	en
dc.subject.other	yeast	en
dc.subject.other	article	en
dc.subject.other	chemistry	en
dc.subject.other	classification	en
dc.subject.other	cluster analysis	en
dc.subject.other	economics	en
dc.subject.other	evaluation	en
dc.subject.other	infrared spectroscopy	en
dc.subject.other	methodology	en
dc.subject.other	mycology	en
dc.subject.other	Pleurotus	en
dc.subject.other	sensitivity and specificity	en
dc.subject.other	time	en
dc.subject.other	Cluster Analysis	en
dc.subject.other	Mycology	en
dc.subject.other	Pleurotus	en
dc.subject.other	Sensitivity and Specificity	en
dc.subject.other	Spectroscopy, Fourier Transform Infrared	en
dc.subject.other	Time Factors	en
dc.subject.other	Agaricales	en
dc.subject.other	Bacteria (microorganisms)	en
dc.subject.other	Basidiomycota	en
dc.subject.other	Fungi	en
dc.subject.other	Mycelium (genus)	en
dc.subject.other	Pleurotus	en
dc.subject.other	Pleurotus djamor	en
dc.subject.other	Pleurotus dryinus	en
dc.subject.other	Pleurotus eryngii	en
dc.subject.other	Pleurotus ostreatus	en
dc.subject.other	Pleurotus pulmonarius	en
dc.title	Rapid strain classification and taxa delimitation within the edible mushroom genus Pleurotus through the use of diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.funbio.2012.04.006	en
heal.publicationDate	2012	en
heal.abstract	Fourier transform infrared (FT-IR) spectroscopy has been successfully applied for the identification of bacteria and yeasts, but only to a limited extent for discriminating specific groups of filamentous fungi. In the frame of this study, 73 strains - from different associated hosts/substrates and geographic regions - representing 16 taxa of the edible mushroom genus Pleurotus (Basidiomycota, Agaricales) were examined through the use of diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy. A binary matrix, elaborated on the basis of presence/absence of specific absorbance peaks combined with cluster analysis, demonstrated that the spectral region 1800-600 cm -1 permitted clear delimitation of individual strains into Pleurotus species. In addition, closely related species (e.g., Pleurotus ostreatus and Pleurotus pulmonarius) or taxa of the subgenus Coremiopleurotus demonstrated high similarity in their absorbance patterns, whereas genetically distinct entities such as Pleurotus dryinus, Pleurotus djamor, and Pleurotus eryngii provided spectra with noteworthy differences. When specific regions (1800-1700, 1360-1285, 1125-1068, and 950-650 cm -1) were evaluated in respect to the absorbance values demonstrated by individual strains, it was evidenced that this methodology could be eventually exploited for the identification of unknown Pleurotus specimens with a stepwise process and with the aid of a dichotomous key developed for this purpose. Moreover, it was shown that the nature of original fungal material examined (mycelium, basidiomata, and basidiospores) had an effect on the outcome of such analyses, and so did the use of different mycelium growth substrates. In conclusion, application of FT-IR spectroscopy provided a fast, reliable, and cost-efficient solution for the classification of pure cultures from closely related mushroom species. © 2012 The British Mycological Society.	en
heal.journalName	Fungal Biology	en
dc.identifier.issue	6	en
dc.identifier.volume	116	en
dc.identifier.doi	10.1016/j.funbio.2012.04.006	en
dc.identifier.spage	715	en
dc.identifier.epage	728	en