Production of 1,3-propanediol, 2,3-butanediol and ethanol by a newly isolated Klebsiella oxytoca strain growing on biodiesel-derived glycerol based media

dc.contributor.author	Metsoviti, M	en
dc.contributor.author	Paraskevaidi, K	en
dc.contributor.author	Koutinas, A	en
dc.contributor.author	Zeng, A-P	en
dc.contributor.author	Papanikolaou, S	en
dc.date.accessioned	2014-06-06T06:51:59Z
dc.date.available	2014-06-06T06:51:59Z
dc.date.issued	2012	en
dc.identifier.issn	13595113	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.procbio.2012.06.011	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5806
dc.subject	1,3-Propanediol	en
dc.subject	2,3-Butanediol	en
dc.subject	Biodiesel	en
dc.subject	Ethanol	en
dc.subject	Klebsiella oxytoca	en
dc.subject	Raw glycerol	en
dc.subject.other	1 ,3 propanediol	en
dc.subject.other	2 ,3-butanediol	en
dc.subject.other	Batch bioreactors	en
dc.subject.other	Co-substrate	en
dc.subject.other	Conversion yield	en
dc.subject.other	Experimental conditions	en
dc.subject.other	Fed-batch fermentation	en
dc.subject.other	Glycerol concentration	en
dc.subject.other	Klebsiella oxytoca	en
dc.subject.other	Metabolic products	en
dc.subject.other	Optimal experimental conditions	en
dc.subject.other	pH condition	en
dc.subject.other	Raw glycerol	en
dc.subject.other	Bacteria	en
dc.subject.other	Biodiesel	en
dc.subject.other	Bioreactors	en
dc.subject.other	Cultivation	en
dc.subject.other	Ethanol	en
dc.subject.other	Fermentation	en
dc.subject.other	Glucose	en
dc.subject.other	Isomers	en
dc.subject.other	Substrates	en
dc.subject.other	Glycerol	en
dc.subject.other	Klebsiella oxytoca	en
dc.title	Production of 1,3-propanediol, 2,3-butanediol and ethanol by a newly isolated Klebsiella oxytoca strain growing on biodiesel-derived glycerol based media	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.procbio.2012.06.011	en
heal.publicationDate	2012	en
heal.abstract	The production of 1,3-propanediol, 2,3-butanediol and ethanol was studied, during cultivations of strain Klebsiella oxytoca FMCC-197 on biodiesel-derived glycerol based media. Different kinds of glycerol feedstocks and experimental conditions had an important impact upon the distribution of metabolic products; production of 1,3-propanediol was positively influenced by stable pH conditions and by the absence of N2 gas infusions throughout the fermentation. Thus, during batch bioreactor fermentations conducted at increasing glycerol concentrations, 1,3-propanediol at 41.3 g/L and yield ∼47% (w/w) was achieved at initial glycerol concentration ∼120 g/L. At even higher initial glycerol media (150 and 170 g/L), growth was not ceased, but 1,3-propanediol production declined. During fed-batch fermentation under optimal experimental conditions, 126 g/L of glycerol were converted into 50.1 g/L of 1,3-propanediol. In this experiment, also 25.2 g/L of ethanol (conversion yield ∼20%, w/w) were formed. A batch-bioreactor culture was performed under non-sterilized conditions and the 1,3-propanediol production was almost equivalent to the sterilized process. Concerning 2,3-butanediol formation, the most detrimental parameter was the absence of N2 sparging and as a result, no 2,3-butanediol was produced. The presence of glucose as co-substrate seriously enhanced 2,3-butanediol production; when commercial glucose was employed as sole substrate, 32.1 g/L of 2,3-butanediol were formed. © 2012 Elsevier Ltd.	en
heal.journalName	Process Biochemistry	en
dc.identifier.issue	12	en
dc.identifier.volume	47	en
dc.identifier.doi	10.1016/j.procbio.2012.06.011	en
dc.identifier.spage	1872	en
dc.identifier.epage	1882	en