Mushroom polysaccharides and lipids synthesized in liquid agitated and static cultures. Part II: Study of volvariella volvacea

dc.contributor.author	Diamantopoulou, P	en
dc.contributor.author	Papanikolaou, S	en
dc.contributor.author	Katsarou, E	en
dc.contributor.author	Komaitis, M	en
dc.contributor.author	Aggelis, G	en
dc.contributor.author	Philippoussis, A	en
dc.date.accessioned	2014-06-06T06:51:56Z
dc.date.available	2014-06-06T06:51:56Z
dc.date.issued	2012	en
dc.identifier.issn	02732289	en
dc.identifier.uri	http://dx.doi.org/10.1007/s12010-012-9714-8	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5776
dc.subject	Biomass	en
dc.subject	Cellular lipids	en
dc.subject	Polysaccharides	en
dc.subject	Submerged cultures	en
dc.subject	Volvariella volvacea	en
dc.subject.other	Agar culture	en
dc.subject.other	Agitated culture	en
dc.subject.other	Biomass productions	en
dc.subject.other	Cellular lipid	en
dc.subject.other	Detailed kinetics	en
dc.subject.other	Dry weight	en
dc.subject.other	Lipid productions	en
dc.subject.other	Liquid culture	en
dc.subject.other	Maximum values	en
dc.subject.other	Mushroom polysaccharides	en
dc.subject.other	Mycelial biomass	en
dc.subject.other	Mycelial growth	en
dc.subject.other	Neutral lipid	en
dc.subject.other	Saturated lipids	en
dc.subject.other	Selected strains	en
dc.subject.other	Static culture	en
dc.subject.other	Submerged cultures	en
dc.subject.other	Total lipids	en
dc.subject.other	Volvariella volvacea	en
dc.subject.other	Biochemistry	en
dc.subject.other	Glucose	en
dc.subject.other	Linoleic acid	en
dc.subject.other	Liquids	en
dc.subject.other	Phospholipids	en
dc.subject.other	Polysaccharides	en
dc.subject.other	Strain	en
dc.subject.other	Biomass	en
dc.subject.other	agar	en
dc.subject.other	glucose	en
dc.subject.other	linoleic acid	en
dc.subject.other	lipid	en
dc.subject.other	phospholipid	en
dc.subject.other	polysaccharide	en
dc.subject.other	article	en
dc.subject.other	biomass	en
dc.subject.other	fatty acid analysis	en
dc.subject.other	fermentation	en
dc.subject.other	fungal strain	en
dc.subject.other	fungus culture	en
dc.subject.other	growth rate	en
dc.subject.other	lipogenesis	en
dc.subject.other	liquid culture	en
dc.subject.other	mushroom	en
dc.subject.other	mycelium	en
dc.subject.other	nonhuman	en
dc.subject.other	Volvariella volvacea	en
dc.subject.other	Biomass	en
dc.subject.other	Cell Culture Techniques	en
dc.subject.other	Culture Media	en
dc.subject.other	Fatty Acids	en
dc.subject.other	Glucose	en
dc.subject.other	Intracellular Space	en
dc.subject.other	Kinetics	en
dc.subject.other	Lipids	en
dc.subject.other	Mycelium	en
dc.subject.other	Polysaccharides	en
dc.subject.other	Time Factors	en
dc.subject.other	Volvariella	en
dc.subject.other	Basidiomycota	en
dc.subject.other	Volvariella volvacea	en
dc.title	Mushroom polysaccharides and lipids synthesized in liquid agitated and static cultures. Part II: Study of volvariella volvacea	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s12010-012-9714-8	en
heal.publicationDate	2012	en
heal.abstract	Volvariella volvacea strains were studied in relation with their ability to produce biomass, lipids and polysaccharides. Firstly, screening of four strains (AMLR 188, 190, 191 and 192) was performed in agar cultures, where the mycelial growth rate of the strains was measured, and in static liquid cultures, where the production of biomass, the biosynthesis of total cellular lipids and the consumption of glucose were monitored. For all strains, biomass production was significant (13-15 gl-1) and total lipid in dry weight (%, w/w) ranged from 3 to 12 %. Afterwards, a detailed kinetic analysis of mycelial biomass, extra- and intracellular polysaccharides (EPS, IPS, respectively) as well as lipid production by a V. volvacea selected strain was conducted in submerged static and agitated cultures. Maximum values of 15 gl-1 biomass, ̃1.0 gl-1 EPS and 5.5 gl-1 IPS were recorded. Agitation did not have severe impact on biomass, EPS and IPS production, but it increased total lipid in dry weight quantities. EPS, IPS and lipid in dry weight values decreased with time. Glucose was the major cellular carbohydrate detected. Total fatty acid analysis of cellular lipids was performed for all V. volvacea strains and linoleic acid δ9,12C18:2 was predominant. Neutral lipids constituted the major fraction of cellular lipids, but their quantity decreased as fermentation proceeded. Phospholipids were the most saturated lipid fraction. © Springer Science+Business Media, LLC 2012.	en
heal.journalName	Applied Biochemistry and Biotechnology	en
dc.identifier.issue	7	en
dc.identifier.volume	167	en
dc.identifier.doi	10.1007/s12010-012-9714-8	en
dc.identifier.spage	1890	en
dc.identifier.epage	1906	en