| dc.contributor.author |
Abell, JL |
en |
| dc.contributor.author |
Garren, JM |
en |
| dc.contributor.author |
Driskell, JD |
en |
| dc.contributor.author |
Tripp, RA |
en |
| dc.contributor.author |
Zhao, Y |
en |
| dc.date.accessioned |
2014-06-06T06:51:53Z |
|
| dc.date.available |
2014-06-06T06:51:53Z |
|
| dc.date.issued |
2012 |
en |
| dc.identifier.issn |
00027863 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1021/ja3043432 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/5755 |
|
| dc.subject.other |
Chemical signatures |
en |
| dc.subject.other |
Label free |
en |
| dc.subject.other |
Label-free detection |
en |
| dc.subject.other |
Least Square |
en |
| dc.subject.other |
Least squares analysis |
en |
| dc.subject.other |
Nucleic acid hybridization |
en |
| dc.subject.other |
Quantitative determinations |
en |
| dc.subject.other |
Relative ratios |
en |
| dc.subject.other |
Reproducibilities |
en |
| dc.subject.other |
SERS substrate |
en |
| dc.subject.other |
Silver nanorods |
en |
| dc.subject.other |
Surface enhanced Raman spectroscopy |
en |
| dc.subject.other |
Nanorods |
en |
| dc.subject.other |
Raman scattering |
en |
| dc.subject.other |
Raman spectroscopy |
en |
| dc.subject.other |
RNA |
en |
| dc.subject.other |
Substrates |
en |
| dc.subject.other |
Surfaces |
en |
| dc.subject.other |
Chemical detection |
en |
| dc.subject.other |
microRNA |
en |
| dc.subject.other |
nanorod |
en |
| dc.subject.other |
nucleotide |
en |
| dc.subject.other |
silver |
en |
| dc.subject.other |
analytic method |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
chemical structure |
en |
| dc.subject.other |
DNA probe |
en |
| dc.subject.other |
quantitative analysis |
en |
| dc.subject.other |
Raman spectrometry |
en |
| dc.subject.other |
regression analysis |
en |
| dc.subject.other |
reproducibility |
en |
| dc.subject.other |
RNA hybridization |
en |
| dc.subject.other |
RNA sequence |
en |
| dc.subject.other |
surface enhanced Raman spectroscopy |
en |
| dc.title |
Label-free detection of micro-RNA hybridization using surface-enhanced raman spectroscopy and least-squares analysis |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1021/ja3043432 |
en |
| heal.publicationDate |
2012 |
en |
| heal.abstract |
Label-free surface-enhanced Raman spectroscopy (SERS) detection of nucleic acid hybridization is impeded by poor spectral reproducibility and the fact that the chemical signatures of hybridized and unhybridized sequences are highly similar. To overcome these issues, highly reproducible silver nanorod SERS substrates along with a straightforward least-squares (LS) technique have been employed for the quantitative determination of the relative ratios of the four nucleotide components A, C, G, and T/U before and after hybridization using a clinically relevant micro-RNA sequence. © 2012 American Chemical Society. |
en |
| heal.journalName |
Journal of the American Chemical Society |
en |
| dc.identifier.issue |
31 |
en |
| dc.identifier.volume |
134 |
en |
| dc.identifier.doi |
10.1021/ja3043432 |
en |
| dc.identifier.spage |
12889 |
en |
| dc.identifier.epage |
12892 |
en |