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Inhibition of A. carbonarius growth and reduction of ochratoxin A by bacteria and yeast composites of technological importance in culture media and beverages

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dc.contributor.author Kapetanakou, AE en
dc.contributor.author Kollias, JN en
dc.contributor.author Drosinos, EH en
dc.contributor.author Skandamis, PN en
dc.date.accessioned 2014-06-06T06:51:51Z
dc.date.available 2014-06-06T06:51:51Z
dc.date.issued 2012 en
dc.identifier.issn 01681605 en
dc.identifier.uri http://dx.doi.org/10.1016/j.ijfoodmicro.2011.09.010 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/5742
dc.subject A. carbonarius en
dc.subject Bacteria en
dc.subject Fermentation en
dc.subject Ochratoxin A en
dc.subject Reduction en
dc.subject Yeasts en
dc.subject.other ochratoxin en
dc.subject.other article en
dc.subject.other Aspergillus carbonarius en
dc.subject.other Bacillus thuringiensis en
dc.subject.other bacterial growth en
dc.subject.other bacterial kinetics en
dc.subject.other bacterial spore en
dc.subject.other bacterial strain en
dc.subject.other bacterium en
dc.subject.other beer en
dc.subject.other beverage en
dc.subject.other cell density en
dc.subject.other concentration (parameters) en
dc.subject.other controlled study en
dc.subject.other culture medium en
dc.subject.other fungus en
dc.subject.other fungus growth en
dc.subject.other fungus spore en
dc.subject.other grape juice en
dc.subject.other growth inhibition en
dc.subject.other Hanseniaspora guilliermondii en
dc.subject.other Hanseniaspora opuntiae en
dc.subject.other Hanseniaspora uvarum en
dc.subject.other incubation time en
dc.subject.other inoculation en
dc.subject.other Issatchekia occidentalis en
dc.subject.other Issatchenkia orientalis en
dc.subject.other Issatchenkia terricola en
dc.subject.other Kazachstania hellenica en
dc.subject.other Kluyveromyces dobzhankii en
dc.subject.other Lachencea thermotolerans en
dc.subject.other Lactobacillus casei en
dc.subject.other Lactobacillus sakei en
dc.subject.other Metschnikowia pulcherrima en
dc.subject.other nonhuman en
dc.subject.other pediococcus pentosaceus en
dc.subject.other pH en
dc.subject.other Pichia fermentas en
dc.subject.other Pichia guilliermondii en
dc.subject.other red wine en
dc.subject.other Saccharomyces cerevisiae en
dc.subject.other Streptococcus salivarius en
dc.subject.other Weissella cibaria en
dc.subject.other yeast en
dc.subject.other zygosaccharomyces bailii en
dc.subject.other Antibiosis en
dc.subject.other Aspergillus en
dc.subject.other Bacteria en
dc.subject.other Beer en
dc.subject.other Biological Control Agents en
dc.subject.other Culture Media en
dc.subject.other Ochratoxins en
dc.subject.other Saccharomyces cerevisiae en
dc.subject.other Temperature en
dc.subject.other Wine en
dc.subject.other Yeasts en
dc.subject.other Aspergillus carbonarius en
dc.subject.other Bacteria (microorganisms) en
dc.subject.other Fungi en
dc.subject.other Vitaceae en
dc.title Inhibition of A. carbonarius growth and reduction of ochratoxin A by bacteria and yeast composites of technological importance in culture media and beverages en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.ijfoodmicro.2011.09.010 en
heal.publicationDate 2012 en
heal.abstract Five composites of yeast and six of bacterial isolates from fermented products were studied, in order to assess their ability to inhibit Aspergillus carbonarius growth and reduce OTA concentration in culture media and beverages. The antagonistic effect of the above composites against A. carbonarius growth was studied in synthetic grape medium of pH 3.5 and a w 0.98, 0.95, 0.92 after incubation at 25°C. Different combinations of initial inocula of bacteria or yeast composites and fungi were used (10 2cfu/mL vs 10 5spores/mL; 10 5cfu/mL vs 10 2spores/mL; and 10 5cfu/mL vs 10 5spores/mL). Regarding the OTA reduction experiment, 10 3 and 10 7cfu/mL of the bacteria and yeast composites were inoculated in liquid media of different pH (3.0, 4.0, 5.0, and 6.1 or 6.5) and initial OTA concentration (50 and 100μg/L) and incubated at 30°C. Moreover, grape juice, red wine, and beer were supplemented with 100μg/L of OTA and inoculated with composites of 16 yeasts (16YM) and 29 bacterial (29BM) strains (10 7cfu/mL) to estimate the kinetics of OTA reduction at 25°C for 5days. Fungal inhibition and OTA reduction were calculated in comparison to control samples. None of the bacterial composites inhibited A. carbonarius growth. The high inoculum of yeast composites (10 5 cfu/mL) showed more efficient fungal inhibition compared to cell density of 10 2 cfu/mL. All yeast composites showed higher OTA reduction (up to 65%) compared to bacteria (2-25%), at all studied assays. The maximum OTA reduction was obtained at pH 3.0 by almost all yeast composites. For all studied beverages the decrease in OTA concentration was higher by yeasts (16YM) compared to bacteria (29BM). The highest OTA reduction was observed in grape juice (ca 32%) followed by wine (ca 22%), and beer (ca 12%). The present findings may assist in the control of A. carbonarius growth and OTA production in fermented foodstuffs by the use of proper strains of technological importance. © 2011 Elsevier B.V. en
heal.journalName International Journal of Food Microbiology en
dc.identifier.issue 3 en
dc.identifier.volume 152 en
dc.identifier.doi 10.1016/j.ijfoodmicro.2011.09.010 en
dc.identifier.spage 91 en
dc.identifier.epage 99 en


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