High Level Resistance against Rhizomania Disease by Simultaneously Integrating Two Distinct Defense Mechanisms

dc.contributor.advisor	Pavli, Ourania I.	en
dc.contributor.advisor	Παυλή, Ουρανία Ι.	el
dc.contributor.author	Tampakaki, Anastasia P.	en
dc.contributor.author	Skaracis, George N.	en
dc.contributor.author	Ταμπακάκη, Αναστασία Π.	el
dc.contributor.author	Σκαράκης, Γεώργιος Ν.	el
dc.date.accessioned	2014-06-06T06:51:50Z
dc.date.available	2014-06-06T06:51:50Z
dc.date.issued	2012-12-20	en
dc.identifier.issn	19326203	en
dc.identifier.uri	http://dx.doi.org/10.1371/journal.pone.0051414	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5727
dc.rights	CC0 1.0 Παγκόσμια	el
dc.rights.uri	http://creativecommons.org/publicdomain/zero/1.0/	en
dc.title	High Level Resistance against Rhizomania Disease by Simultaneously Integrating Two Distinct Defense Mechanisms	en
heal.type	journalArticle	en
heal.keyword	Genetic transformation	en
heal.keyword	Inoculation	en
heal.keyword	Nicotiana benthamiana	en
heal.keyword	Plant defense	en
heal.keyword	Plant disease	en
heal.keyword	Plant genetics	en
heal.keyword	Plant virus	en
heal.keyword	Rhizomania disease	en
heal.keyword	Transgenic plant	en
heal.keyword	Beta vulgaris	en
heal.keyword	Virus resistance	en
heal.keyword	Virus isolation	en
heal.keyword	Protein secretion	en
heal.keyword	Progeny	en
heal.keyword	Sugar beet	en
heal.identifier.primary	10.1371/journal.pone.0051414	en
heal.identifier.secondary	e51414	en
heal.recordProvider	Γεωπονικό Πανεπιστήμιο Αθηνών/Επιστήμη Φυτικής Παραγωγής	el
heal.recordProvider	Γεωπονικό Πανεπιστήμιο Αθηνών/Επιστήμη Γεωπονικής Βιοτεχνολογίας	el
heal.publicationDate	2012-12-20	en
heal.bibliographicCitation	Pavli, Ourania I. High Level Resistance against Rhizomania Disease by Simultaneously Integrating Two Distinct Defense Mechanisms, PLoS One, vol.7 (12), pp 1-6, PLOS 2012	en
heal.abstract	With the aim of achieving durable resistance against rhizomania disease of sugar beet, the employment of different sources of resistance to Beet necrotic yellow vein virus was pursued. To this purpose, Nicotiana benthamiana transgenic plants that simultaneously produce dsRNA originating from a conserved region of the BNYVV replicase gene and the HrpZPsph protein in a secreted form (SP/HrpZPsph) were produced. The integration and expression of both transgenes as well as proper production of the harpin protein were verified in all primary transformants and selfed progeny (T1, T2). Transgenic resistance was assessed by BNYVV-challenge inoculation on T2 progeny by scoring disease symptoms and DAS-ELISA at 20 and 30 dpi. Transgenic lines possessing single transformation events for both transgenes as well as wild type plants were included in inoculation experiments. Transgenic plants were highly resistant to virus infection, whereas in some cases immunity was achieved. In all cases, the resistant phenotype of transgenic plants carrying both transgenes was superior in comparison with the ones carrying a single transgene. Collectively, our findings demonstrate, for a first time, that the combination of two entirely different resistance mechanisms provide high level resistance or even immunity against the virus. Such a novel approach is anticipated to prevent a rapid virus adaptation that could potentially lead to the emergence of isolates with resistance breaking properties. © 2012 Pavli et al.	en
heal.publisher	Public Library of Science (PLoS)	en
heal.journalName	PLoS ONE	en
dc.identifier.doi	10.1371/journal.pone.0051414	en