| dc.contributor.author |
Zambounis, AG |
en |
| dc.contributor.author |
Kalamaki, MS |
en |
| dc.contributor.author |
Tani, EE |
en |
| dc.contributor.author |
Paplomatas, EJ |
en |
| dc.contributor.author |
Tsaftaris, AS |
en |
| dc.date.accessioned |
2014-06-06T06:51:48Z |
|
| dc.date.available |
2014-06-06T06:51:48Z |
|
| dc.date.issued |
2012 |
en |
| dc.identifier.issn |
07359640 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1007/s11105-011-0335-0 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/5706 |
|
| dc.subject |
Chitinase (PR-3) |
en |
| dc.subject |
Defense reactions |
en |
| dc.subject |
Glutathione S-Transferase (GST18) |
en |
| dc.subject |
Real-time PCR |
en |
| dc.subject |
Ribonuclease-like (PR-10) |
en |
| dc.subject.other |
Fusarium oxysporum |
en |
| dc.subject.other |
Fusarium oxysporum f. sp. vasinfectum |
en |
| dc.subject.other |
Gossypium hirsutum |
en |
| dc.title |
Expression Analysis of Defense-Related Genes in Cotton (Gossypium hirsutum) after Fusarium oxysporum f. sp. vasinfectum Infection and Following Chemical Elicitation using a Salicylic Acid Analog and Methyl Jasmonate |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1007/s11105-011-0335-0 |
en |
| heal.publicationDate |
2012 |
en |
| heal.abstract |
Cotton (Gossypium hirsutum) wilt caused by Fusarium oxysporum f. sp. vasinfectum (Fov) is considered as a major threat for commercial cotton production worldwide. Relative expression ratios of two key pathogenesis-related (PR) genes (PR-3 and PR-10) and a detoxification gene (GST18) were compared between a fully susceptible (""LACTA"") and a partially field-resistant (""EMERALD"") cultivar after challenging with an Australian Fov isolate, as well as after pre-treatments with chemical inducers of defense such as BION® (a chemical analog of salicylic acid) and methyl-jasmonate (MeJA) prior to Fov inoculation. It was demonstrated that in both hypocotyls and roots of ""EMERALD"", all PR genes were over-expressed after inoculation with Fov but not in the fully susceptible cultivar. Fov inoculation did not significantly affect GST18 expression in both cultivars. Exogenous application of each defense elicitor, prior to Fov inoculation, resulted in up-regulation of the three genes in root tissues of the fully susceptible cultivar. BION® application did not influence PR-3 expression in hypocotyls of both cultivars, whereas MeJA application resulted in induction of PR-3 in both cultivars. Furthermore, in hypocotyls of ""LACTA"", over-expression of PR-10 was recorded after treatment with each chemical inducer. This pathogen exhibited different ability in eliciting oxidative burst in roots of the two cotton cultivars used in our analysis. © 2011 Springer-Verlag. |
en |
| heal.journalName |
Plant Molecular Biology Reporter |
en |
| dc.identifier.issue |
1 |
en |
| dc.identifier.volume |
30 |
en |
| dc.identifier.doi |
10.1007/s11105-011-0335-0 |
en |
| dc.identifier.spage |
225 |
en |
| dc.identifier.epage |
234 |
en |