Evaluation of bioefficacy of three Citrus essential oils against the dengue vector Aedes albopictus (Diptera: Culicidae) in correlation to their components enantiomeric distribution

dc.contributor.author	Giatropoulos, A	en
dc.contributor.author	Papachristos, DP	en
dc.contributor.author	Kimbaris, A	en
dc.contributor.author	Koliopoulos, G	en
dc.contributor.author	Polissiou, MG	en
dc.contributor.author	Emmanouel, N	en
dc.contributor.author	Michaelakis, A	en
dc.date.accessioned	2014-06-06T06:51:47Z
dc.date.available	2014-06-06T06:51:47Z
dc.date.issued	2012	en
dc.identifier.issn	09320113	en
dc.identifier.uri	http://dx.doi.org/10.1007/s00436-012-3074-8	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5692
dc.subject.other	beta citral	en
dc.subject.other	beta pinene	en
dc.subject.other	citral	en
dc.subject.other	citrus oil	en
dc.subject.other	insect repellent	en
dc.subject.other	larvicidal agent	en
dc.subject.other	limonene	en
dc.subject.other	terpinene	en
dc.subject.other	unclassified drug	en
dc.subject.other	Aedes albopictus	en
dc.subject.other	animal experiment	en
dc.subject.other	arthropod larva	en
dc.subject.other	article	en
dc.subject.other	chemical composition	en
dc.subject.other	controlled study	en
dc.subject.other	enantiomer	en
dc.subject.other	female	en
dc.subject.other	gas chromatography	en
dc.subject.other	grapefruit	en
dc.subject.other	imago	en
dc.subject.other	LC 50	en
dc.subject.other	lemon	en
dc.subject.other	male	en
dc.subject.other	nonhuman	en
dc.subject.other	priority journal	en
dc.subject.other	sweet orange	en
dc.subject.other	Aedes	en
dc.subject.other	Animals	en
dc.subject.other	Biological Assay	en
dc.subject.other	Chromatography, Gas	en
dc.subject.other	Citrus	en
dc.subject.other	Disease Vectors	en
dc.subject.other	Insect Repellents	en
dc.subject.other	Insecticides	en
dc.subject.other	Isomerism	en
dc.subject.other	Larva	en
dc.subject.other	Oils, Volatile	en
dc.subject.other	Plant Extracts	en
dc.subject.other	Survival Analysis	en
dc.subject.other	Aedes albopictus	en
dc.subject.other	Citrus	en
dc.subject.other	Citrus limon	en
dc.subject.other	Citrus sinensis	en
dc.subject.other	Culicidae	en
dc.subject.other	Diptera	en
dc.title	Evaluation of bioefficacy of three Citrus essential oils against the dengue vector Aedes albopictus (Diptera: Culicidae) in correlation to their components enantiomeric distribution	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s00436-012-3074-8	en
heal.publicationDate	2012	en
heal.abstract	Laboratory experiments were conducted to study the bioefficacy against Ae. albopictus of three Citrus essential oils, derived from peels of Citrus sinensis, Citrus limon, and Citrus paradise and of their components. Chiral gas chromatography analysis revealed the dominant occurrence of R-(+)- limonene and (-)-β-pinene in all three essential oils while in the case of lemon oil γ-terpinene, neral, and geranial detected also among other components. The tested Citrus essential oils were toxic against mosquito larvae with LC 50 values ranging from 25.03 to 37.03 mgl-1. Among citrus essential oils components tested, γ-terpinene was the most toxic (LC 50=20.21 mgl-1) followed by both enantiomeric forms of limonene (LC50=35.99 and 34.89 mgl-1, for R-(+)-limonene and S-(-)-limonene, respectively). The delayed toxic effects after exposure of larvae to sublethal (LC50) doses were also investigated for citrus essential oils and their major component R-(+)-limonene, indicating a significant reduction of pupal survival. In repellent bioassays, lemon essential oil, S-(-)- limonene, citral (mixture of neral\geranial) and (+)-β-pinene were the most effective compared with other citrus essential oils and components against adult mosquitoes. Repellent bioassays also revealed that limonenes and β-pinenes showed an isomer dependence repellent activity. Finally, according to enantiomeric distribution of limonene and α- and β-pinene, the repellency of lemon essential oil is possibly attributed to the presence of citral. © Springer-Verlag 2012.	en
heal.journalName	Parasitology Research	en
dc.identifier.issue	6	en
dc.identifier.volume	111	en
dc.identifier.doi	10.1007/s00436-012-3074-8	en
dc.identifier.spage	2253	en
dc.identifier.epage	2263	en