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Estimating the diagnostic accuracy of three culture-dependent methods for the Listeria monocytogenes detection from a Bayesian perspective

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dc.contributor.author Andritsos, ND en
dc.contributor.author Mataragas, M en
dc.contributor.author Paramithiotis, S en
dc.contributor.author Nychas, GJE en
dc.contributor.author Drosinos, EH en
dc.date.accessioned 2014-06-06T06:51:46Z
dc.date.available 2014-06-06T06:51:46Z
dc.date.issued 2012 en
dc.identifier.issn 01681605 en
dc.identifier.uri http://dx.doi.org/10.1016/j.ijfoodmicro.2012.03.027 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/5689
dc.subject Accuracy measures en
dc.subject Bayesian analysis en
dc.subject Diagnostic tests en
dc.subject Dirichlet distribution en
dc.subject Listeria monocytogenes en
dc.subject Pork meat en
dc.subject.other area under the curve en
dc.subject.other article en
dc.subject.other bacterium culture en
dc.subject.other bacterium detection en
dc.subject.other Bayes theorem en
dc.subject.other controlled study en
dc.subject.other culture medium en
dc.subject.other diagnostic test en
dc.subject.other food contamination en
dc.subject.other Listeria monocytogenes en
dc.subject.other meat en
dc.subject.other multivariate analysis en
dc.subject.other nonhuman en
dc.subject.other pork en
dc.subject.other predictive value en
dc.subject.other receiver operating characteristic en
dc.subject.other sensitivity and specificity en
dc.subject.other statistical model en
dc.subject.other Animals en
dc.subject.other Area Under Curve en
dc.subject.other Bayes Theorem en
dc.subject.other Commerce en
dc.subject.other Food Microbiology en
dc.subject.other Humans en
dc.subject.other Listeria monocytogenes en
dc.subject.other Meat en
dc.subject.other Predictive Value of Tests en
dc.subject.other ROC Curve en
dc.subject.other Sensitivity and Specificity en
dc.subject.other Swine en
dc.subject.other Listeria monocytogenes en
dc.title Estimating the diagnostic accuracy of three culture-dependent methods for the Listeria monocytogenes detection from a Bayesian perspective en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.ijfoodmicro.2012.03.027 en
heal.publicationDate 2012 en
heal.abstract The objective of this study was to estimate the test accuracy measures (classification probabilities [CPs], predictive values [PVs], likelihood ratios [LRs] and area under receiving operating characteristic curve [AUC]) of three different culture-dependent methods, commonly used during routine analysis for the detection of the foodborne pathogen Listeria monocytogenes, from a Bayesian perspective. Data from a previous study by Andritsos et al. (2010) were used to define measures of accuracy for the diagnostic tests. Samples of minced pork meat obtained from local markets were tested for L. monocytogenes presence by parallel testing using selective media (PALCAM, ALOA and RAPID'L.mono). Dirichlet distribution, which is the multivariate expression of a Beta distribution, was used to analyze the data. Bayesian analysis determines characteristics of the posterior distribution from available prior information. Results showed that all methods were best at ruling in L. monocytogenes presence than ruling it out. PALCAM seemed to have better performance based on positive PV, positive LR and AUC, but it was not so sensitive as RAPID'L.mono was. Results also showed that none of the media were perfect in detecting L. monocytogenes, i.e. sensitivity and specificity equal to one. Besides, the problem of observing zero counts may be overcome by applying Bayesian analysis, making the determination of a test performance feasible. © 2012 Elsevier B.V. en
heal.journalName International Journal of Food Microbiology en
dc.identifier.issue 2 en
dc.identifier.volume 156 en
dc.identifier.doi 10.1016/j.ijfoodmicro.2012.03.027 en
dc.identifier.spage 181 en
dc.identifier.epage 185 en


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