| dc.contributor.author |
Michail, X |
en |
| dc.contributor.author |
Kontogiannatos, D |
en |
| dc.contributor.author |
Syriou, V |
en |
| dc.contributor.author |
Kourti, A |
en |
| dc.date.accessioned |
2014-06-06T06:51:40Z |
|
| dc.date.available |
2014-06-06T06:51:40Z |
|
| dc.date.issued |
2012 |
en |
| dc.identifier.issn |
09639292 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1007/s10646-012-0980-6 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/5625 |
|
| dc.subject |
Bisphenol A |
en |
| dc.subject |
Developmental progression |
en |
| dc.subject |
Hsc70 |
en |
| dc.subject |
Hsp83 |
en |
| dc.subject |
Sesamia nonagrioides |
en |
| dc.subject |
SHsps |
en |
| dc.subject.other |
4,4' isopropylidenediphenol |
en |
| dc.subject.other |
heat shock protein |
en |
| dc.subject.other |
messenger RNA |
en |
| dc.subject.other |
sex hormone |
en |
| dc.subject.other |
endocrine system |
en |
| dc.subject.other |
larva |
en |
| dc.subject.other |
metamorphosis |
en |
| dc.subject.other |
phenotype |
en |
| dc.subject.other |
polymerase chain reaction |
en |
| dc.subject.other |
protein |
en |
| dc.subject.other |
RNA |
en |
| dc.subject.other |
vertebrate |
en |
| dc.subject.other |
animal experiment |
en |
| dc.subject.other |
animal tissue |
en |
| dc.subject.other |
arthropod larva |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
artificial diet |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
developmental stage |
en |
| dc.subject.other |
developmental toxicity |
en |
| dc.subject.other |
feeding |
en |
| dc.subject.other |
gene |
en |
| dc.subject.other |
gene expression |
en |
| dc.subject.other |
genotoxicity |
en |
| dc.subject.other |
heat shock protein gene |
en |
| dc.subject.other |
injection |
en |
| dc.subject.other |
metamorphosis |
en |
| dc.subject.other |
molting |
en |
| dc.subject.other |
moth |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
phenotype |
en |
| dc.subject.other |
priority journal |
en |
| dc.subject.other |
real time polymerase chain reaction |
en |
| dc.subject.other |
RNA synthesis |
en |
| dc.subject.other |
Sesamia nonagrioides |
en |
| dc.subject.other |
SnoHsp19.5 gene |
en |
| dc.subject.other |
SnoHsp20.8 gene |
en |
| dc.subject.other |
SnoHsp70 gene |
en |
| dc.subject.other |
SnoHsp83 gene |
en |
| dc.subject.other |
terrestrial species |
en |
| dc.subject.other |
transcription regulation |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
Benzhydryl Compounds |
en |
| dc.subject.other |
Dose-Response Relationship, Drug |
en |
| dc.subject.other |
Estrogens, Non-Steroidal |
en |
| dc.subject.other |
Gene Expression Regulation |
en |
| dc.subject.other |
Heat-Shock Proteins |
en |
| dc.subject.other |
Insect Proteins |
en |
| dc.subject.other |
Larva |
en |
| dc.subject.other |
Moths |
en |
| dc.subject.other |
Phenols |
en |
| dc.subject.other |
Pupa |
en |
| dc.subject.other |
Real-Time Polymerase Chain Reaction |
en |
| dc.subject.other |
Hexapoda |
en |
| dc.subject.other |
Lepidoptera |
en |
| dc.subject.other |
Noctuidae |
en |
| dc.subject.other |
Sesamia nonagrioides |
en |
| dc.subject.other |
Vertebrata |
en |
| dc.title |
Bisphenol-A affects the developmental progression and expression of heat-shock protein genes in the moth Sesamia nonagrioides |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1007/s10646-012-0980-6 |
en |
| heal.publicationDate |
2012 |
en |
| heal.abstract |
The effects of bisphenol A (BPA) on the endocrine system of vertebrates have been demonstrated in several studies. Here, we report the impact of BPA on the developmental progression and expression of heat shock protein genes on the terrestrial insect Sesamia nonagrioides (Lepidoptera: Noctuidae). S. nonagrioides 1st instar larvae were exposed until the end of 6th (last) instar to selected concentrations of BPA (1 μg/L, 10 μg/L, 100 μg/L, 1 mg/L and 10 mg/L) applied in their artificial diets. The lower doses of BPA (1-10 μg/L) were found to decrease larvae's weight while the 100 μg/L dose increased it. The higher doses of BPA were found to induce various abnormal phenotypes during 5th instar larval molting, larval-pupal transformation and metamorphosis. The developmental and metamorphosis endpoints presented here may indicate the possible impact of BPA on terrestrial insects. Additionally, 6th instar larvae were injected with several concentrations of BPA. Semi-quantitative and Real-Time PCR assays were used to identify the effects of BPA in the transcriptional regulation of five heat shock protein genes (SnoHsp19.5, SnoHsp20.8, SnoHsp70, SnoHsc70 and SnoHsp83). Application of BPA by feeding or by injection induced the synthesis of the SnoHsp19.5 and SnoHsp20.8 mRNAs. The expression levels of SnoHsp70 were not affected. In contrast, SnoHsc70 and SnoHsp83, which play a pivotal role in vertebrate sex steroid signal transduction, were elevated by BPA. Our results suggest that SnoHsp19.5, SnoHsp20.8, SnoHsp83 and SnoHsc70 genes can be modulated by BPA. © Springer Science+Business Media, LLC 2012. |
en |
| heal.journalName |
Ecotoxicology |
en |
| dc.identifier.issue |
8 |
en |
| dc.identifier.volume |
21 |
en |
| dc.identifier.doi |
10.1007/s10646-012-0980-6 |
en |
| dc.identifier.spage |
2244 |
en |
| dc.identifier.epage |
2253 |
en |