Defined media for optimal pyoverdine production by Pseudomonas fluorescens 2-79

dc.contributor.author	Kisaalita, WS	en
dc.contributor.author	Slininger, PJ	en
dc.contributor.author	Bothast, RJ	en
dc.date.accessioned	2014-06-06T06:42:20Z
dc.date.available	2014-06-06T06:42:20Z
dc.date.issued	1993	en
dc.identifier.issn	01757598	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/561
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-0027244752&partnerID=40&md5=e2a2ff34bc1405b9fd85900e983dad53	en
dc.subject.other	1 phenazinecarboxylic acid	en
dc.subject.other	glutamic acid	en
dc.subject.other	pyoverdine	en
dc.subject.other	unclassified drug	en
dc.subject.other	antibiotic biosynthesis	en
dc.subject.other	article	en
dc.subject.other	biomass	en
dc.subject.other	nonhuman	en
dc.subject.other	pseudomonas fluorescens	en
dc.title	Defined media for optimal pyoverdine production by Pseudomonas fluorescens 2-79	en
heal.type	journalArticle	en
heal.publicationDate	1993	en
heal.abstract	Pseudomonas fluorescens strain 2-79 (NRRL-15132) produces a fluorescent yellow-green pyoverdine when cultured on Fe(III)-poor medium. When cultured on Fe(III)-rich medium, strain 2-79 produces an antibiotic, phenazine 1-carboxylic acid, which is effective in suppressing plant fungal diseases such as take-all of wheat. A 23 factorial design was used to examine pyoverdine production as a function of the presence or absence of Bacto casamino acids, purines-pyrimidines and vitamins in an iron-deficient medium. Amino acids were found to be an important factor (P = 0.0002). A Plackett-Burman design was used to identify eight amino acids, out of the 19 present in casamino acids, that were responsible for the increased pyoverdine production: methionine, valine, isoleucine, tyrosine, proline, phenylalanine, glutamic acid, and glycine. Biomass was enhanced only by glutamic acid.	en
heal.journalName	Applied Microbiology and Biotechnology	en
dc.identifier.issue	6	en
dc.identifier.volume	39	en
dc.identifier.spage	750	en
dc.identifier.epage	755	en